Oncotarget

Research Papers:

LKB1 promotes cell survival by modulating TIF-IA-mediated pre-ribosomal RNA synthesis under uridine downregulated conditions

Fakeng Liu _, Rui Jin, Xiuju Liu, Henry Huang, Scott C. Wilkinson, Diansheng Zhong, Fadlo R. Khuri, Haian Fu, Adam Marcus, Yulong He and Wei Zhou

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Oncotarget. 2016; 7:2519-2531. https://doi.org/10.18632/oncotarget.6224

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Abstract

Fakeng Liu1,2,*, Rui Jin1,*, Xiuju Liu1, Henry Huang1, Scott C. Wilkinson1,3, Diansheng Zhong1,4, Fadlo R. Khuri1, Haian Fu5, Adam Marcus1, Yulong He2 and Wei Zhou1,6

1 Department of Hematology and Medical Oncology, Emory University School of Medicine, Atlanta, GA, USA

2 Department of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, P.R. China

3 Graduate Program in Cancer Biology, Emory University, Atlanta, GA, USA

4 Department of Medical Oncology, Tianjin Medical University General Hospital, Tianjin, P.R.China

5 Department of Pharmacology, Emory University School of Medicine, Atlanta, GA, USA

6 Department of Pathology and Laboratory Medicine and Department of Human Genetics Emory University School of Medicine, Atlanta, GA, USA

* These authors have contributed equally to this work

Correspondence to:

Wei Zhou, email:

Keywords: pre-ribosomal RNA synthesis, TIF-IA, serine/threonine kinase 11, targeted therapy, tumor suppressor

Received: July 29, 2015 Accepted: October 09, 2015 Published: October 25, 2015

Abstract

We analyzed the mechanism underlying 5-aminoimidazole-4-carboxamide riboside (AICAR) mediated apoptosis in LKB1-null non-small cell lung cancer (NSCLC) cells. Metabolic profile analysis revealed depletion of the intracellular pyrimidine pool after AICAR treatment, but uridine was the only nucleotide precursor capable of rescuing this apoptosis, suggesting the involvement of RNA metabolism. Because half of RNA transcription in cancer is for pre-ribosomal RNA (rRNA) synthesis, which is suppressed by over 90% after AICAR treatment, we evaluated the role of TIF-IA-mediated rRNA synthesis. While the depletion of TIF-IA by RNAi alone promoted apoptosis in LKB1-null cells, the overexpression of a wild-type or a S636A TIF-IA mutant, but not a S636D mutant, attenuated AICAR-induced apoptosis. In LKB1-null H157 cells, pre-rRNA synthesis was not suppressed by AICAR when wild-type LKB1 was present, and cellular fractionation analysis indicated that TIF-IA quickly accumulated in the nucleus in the presence of a wild-type LKB1 but not a kinase-dead mutant. Furthermore, ectopic expression of LKB1 was capable of attenuating AICAR-induced death in AMPK-null cells. Because LKB1 promotes cell survival by modulating TIF-IA-mediated pre-rRNA synthesis, this discovery suggested that targeted depletion of uridine related metabolites may be exploited in the clinic to eliminate LKB1-null cancer cells.


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