Oncotarget

Research Papers:

Silencing P2X7 receptor downregulates the expression of TCP-1 involved in lymphoma lymphatic metastasis

Xudong Jiang, Wenjuan Mao, Ziyi Yang, Jia Zeng, Yi Zhang, Yang Song, Ying Kong, Shuangyi Ren and Yunfei Zuo _

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Oncotarget. 2015; 6:42105-42117. https://doi.org/10.18632/oncotarget.5870

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Abstract

Xudong Jiang1,*, Wenjuan Mao1,*, Ziyi Yang1, Jia Zeng1, Yi Zhang1,2, Yang Song1,2, Ying Kong3, Shuangyi Ren2, Yunfei Zuo1

1Department of Clinical Biochemistry, College of Laboratory Diagnostic Medicine, Dalian Medical University, Dalian 116044, China

2Department of Surgery, the Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China

3Department of Biochemistry, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, China

*These authors have equally contributed to this work

Correspondence to:

Yunfei Zuo, e-mail: [email protected]

Keywords: P2X7R, TCP-1, lymphoma, proteomics, lymphatic-metastasis

Received: April 18, 2015     Accepted: October 27, 2015     Published: November 06, 2015

ABSTRACT

P2X7R is an ATP-gated cation channel that participates in cell proliferation and apoptosis. TCP-1 assists with the protein folding. According to our previous research, the P2X7R has a potential role in P388D1 lymphoid neoplasm cells dissemination to peripheral lymph nodes. In order to make a further exploration about the probable mechanism, the lymph nodes which metastasized by P2X7R-silenced P388D1 cells or non-silenced cells were analyzed by 2DE and a MALDI-TOF-based proteomics approach. In the 64 proteins which were differentially expressed between two groups, TCP-1 was found to be significantly decreased in P2X7R shRNA group compared to controls. This correlation was also found in subsequent experiments in vivo and in vitro. The positive correlation between P2X7R and TCP-1 was also proved in both lymphoma and benign lymphadenopathy tissues from patients. It indicates that TCP-1 may be a crucial downstream molecular of P2X7R and plays a novel role in lymphoid neoplasm metastasis.


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