The effective bioengineering method of implantation decellularized renal extracellular matrix scaffolds
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Yong Guan1, Shuangde Liu2, Chao Sun3, Guanghui Cheng3, Feng Kong3, Yun Luan3, Xiaoshuai Xie1, Shengtian Zhao1, Denglu Zhang1, Jue Wang3, Kailin Li3, Yuqiang Liu1
1The Second Hospital, Shandong University, Department of Urology, Shandong, China
2The Second Hospital, Shandong University, Department of Kidney Transplantation, Shandong, China
3The Second Hospital, Shandong University, Department of Central Research Lab, Shandong, China
Shengtian Zhao, e-mail: email@example.com
Keywords: scaffold, tissue engineering, kidney regeneration, decellularization, recellularization
Received: March 16, 2015 Accepted: September 11, 2015 Published: September 21, 2015
End stage renal disease (ESRD) is a progressive loss of kidney function with a high rate of morbidity and mortality. Transplantable organs are hard to come by and hold a high risk of recipient immune rejection. We intended to establish a more effective and faster method to decellularize and recellularize the kidney scaffold for transplant and regeneration. We successfully produced renal scaffolds by decellularizing rat kidneys with 0.5% sodium dodecyl sulfate (SDS), while still preserving the extracellular matrix (ECM) 3D architecture, an intact vascular tree and biochemical components. We recellularized the kidney scaffolds with mouse embryonic stem (ES) cells that then populated and proliferated within the glomerular, vascular, and tubular structures. After in vivo implantation, these recellularized scaffolds were easily reperfused, tolerated blood pressure and produced urine with no blood leakage. Our methods can successfully decellularize and recellularize rat kidneys to produce functional renal ECM scaffolds. These scaffolds maintain their basic components, retain intact vasculature and show promise for kidney regeneration.
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