Twist promotes reprogramming of glucose metabolism in breast cancer cells through PI3K/AKT and p53 signaling pathways

Li Yang; Yixuan Hou; Jie Yuan; Shifu Tang; Hailong Zhang; Qing Zhu; Yan-e Du; Mingli Zhou; Siyang Wen; Liyun Xu; Xi Tang; Xiaojiang Cui; Manran Liu

doi:10.18632/oncotarget.4697

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Research Papers:

Twist promotes reprogramming of glucose metabolism in breast cancer cells through PI3K/AKT and p53 signaling pathways

Li Yang, Yixuan Hou, Jie Yuan, Shifu Tang, Hailong Zhang, Qing Zhu, Yan-e Du, Mingli Zhou, Siyang Wen, Liyun Xu, Xi Tang, Xiaojiang Cui and Manran Liu _

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Oncotarget. 2015; 6:25755-25769. https://doi.org/10.18632/oncotarget.4697

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Abstract

Li Yang1,*, Yixuan Hou2,*, Jie Yuan3, Shifu Tang1, Hailong Zhang1, Qing Zhu3, Yan-e Du1, Mingli Zhou1, Siyang Wen1, Liyun Xu1, Xi Tang1, Xiaojiang Cui4, Manran Liu1

1Key Laboratory of Laboratory Medical Diagnostics, Chinese Ministry of Education, Chongqing Medical University, Chongqing 400016, China

2Experimental Teaching Center of Basic Medicine Science, Chongqing Medical University, Chongqing 400016, China

3Department of Endocrine and Breast Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

4Department of Surgery, Department of Obstetrics and Gynecology, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA

*These authors have contributed equally to this work

Correspondence to:

Manran Liu, e-mail: [email protected]

Keywords: glucose metabolism, Twist, PI3K/AKT, p53

Received: February 15, 2015 Accepted: July 08, 2015 Published: July 27, 2015

ABSTRACT

Twist, a key regulator of epithelial-mesenchymal transition (EMT), plays an important role in the development of a tumorigenic phenotype. Energy metabolism reprogramming (EMR), a newly discovered hallmark of cancer cells, potentiates cancer cell proliferation, survival, and invasion. Currently little is known about the effects of Twist on tumor EMR. In this study, we found that glucose consumption and lactate production were increased and mitochondrial mass was decreased in Twist-overexpressing MCF10A mammary epithelial cells compared with vector-expressing MCF10A cells. Moreover, these Twist-induced phenotypic changes were augmented by hypoxia. The expression of some glucose metabolism-related genes such as PKM2, LDHA, and G6PD was also found to be upregulated. Mechanistically, activated β1-integrin/FAK/PI3K/AKT/mTOR and suppressed P53 signaling were responsible for the observed EMR. Knockdown of Twist reversed the effects of Twist on EMR in Twist-overexpressing MCF10A cells and Twist-positive breast cancer cells. Furthermore, blockage of the β1-integrin/FAK/PI3K/AKT/mTOR pathway by siRNA or specific chemical inhibitors, or rescue of p53 activation can partially reverse the switch of glucose metabolism and inhibit the migration of Twist-overexpressing MCF10A cells and Twist-positive breast cancer cells. Thus, our data suggest that Twist promotes reprogramming of glucose metabolism in MCF10A-Twist cells and Twist-positive breast cancer cells via activation of the β1-integrin/FAK/PI3K/AKT/mTOR pathway and inhibition of the p53 pathway. Our study provides new insight into EMR.

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Research Papers:

Twist promotes reprogramming of glucose metabolism in breast cancer cells through PI3K/AKT and p53 signaling pathways

Abstract