Intron-based genomic editing: a highly efficient method for generating knockin zebrafish
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Jia Li1, Baibing Zhang1,3, Jiwen Bu1 and Jiulin Du1,2,3
1 Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
2 School of Life Science and Technology, ShanghaiTech University, Shanghai 200031, China
3 University of Chinese Academy of Sciences, Shanghai 200031, China
Jia Li, email:
Jiulin Du, email:
Keywords: knockin, CRISPR/Cas9, zebrafish
Received: May 27, 2015 Accepted: June 06, 2015 Published: June 19, 2015
The TALEN and CRISPR/Cas9 nuclease systems have been extensively utilized in genomic engineering of model organisms. In zebrafish, the nuclease systems have been successfully applied in generating loss-of–function knockout lines. However, genome-specific knockin techniques in zebrafish are still at the beginning. In this perspective, we briefly summarize the recent progresses on knockin approaches in zebrafish with a special focus on the newly developed intron-based knockin method.
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