Oncotarget

Research Perspectives:

Intron-based genomic editing: a highly efficient method for generating knockin zebrafish

Jia Li _, Baibing Zhang, Jiwen Bu and Jiulin Du

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Oncotarget. 2015; 6:17891-17894. https://doi.org/10.18632/oncotarget.4547

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Abstract

Jia Li1, Baibing Zhang1,3, Jiwen Bu1 and Jiulin Du1,2,3

1 Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

2 School of Life Science and Technology, ShanghaiTech University, Shanghai 200031, China

3 University of Chinese Academy of Sciences, Shanghai 200031, China

Correspondence to:

Jia Li, email:

Jiulin Du, email:

Keywords: knockin, CRISPR/Cas9, zebrafish

Received: May 27, 2015 Accepted: June 06, 2015 Published: June 19, 2015

Abstract

The TALEN and CRISPR/Cas9 nuclease systems have been extensively utilized in genomic engineering of model organisms. In zebrafish, the nuclease systems have been successfully applied in generating loss-of–function knockout lines. However, genome-specific knockin techniques in zebrafish are still at the beginning. In this perspective, we briefly summarize the recent progresses on knockin approaches in zebrafish with a special focus on the newly developed intron-based knockin method.


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