Antibody and lectin target podoplanin to inhibit oral squamous carcinoma cell migration and viability by distinct mechanisms
Metrics: PDF 1879 views | HTML 35241 views | ?
Jhon A. Ochoa-Alvarez1, Harini Krishnan1, John G. Pastorino1, Evan Nevel1, David Kephart1, Joseph J. Lee1, Edward P. Retzbach1, Yongquan Shen1, Mahnaz Fatahzadeh2, Soly Baredes3, Evelyne Kalyoussef3, Masaru Honma4, Martin E. Adelson5, Mika K. Kaneko6, Yukinari Kato6, Mary Ann Young1, Lisa Deluca-Rapone1, Alan J. Shienbaum1, Kingsley Yin1, Lasse D. Jensen7 and Gary S. Goldberg1
1 Departments of Molecular Biology, Cell Biology, and Pathology, School of Osteopathic Medicine, Rowan University, Stratford, NJ, USA
2 Department of Diagnostic Sciences, Rutgers School of Dental Medicine, Newark, NJ, USA
3 Department of Otolaryngology - Head and Neck Surgery, Rutgers New Jersey Medical School, Newark, NJ, USA
4 Department of Dermatology, Asahikawa Medical University, Midorigaoka-Higashi, Asahikawa, Japan
5 Medical Diagnostic Laboratories, Hamilton, NJ, USA
6 Department of Regional Innovation, Tohoku University Graduate School of Medicine, Seiryo-machi, Aoba-ku, Sendai, Miyagi, Japan
7 Department of Medical and Health Sciences, Linköping University, Lasarettsgatan, Ingång, Linköping, Sweden
Gary S. Goldberg, email:
Keywords: podoplanin, cancer, cell migration, receptor, lectin
Received: October 13, 2014 Accepted: February 04, 2015 Published: March 10, 2015
Podoplanin (PDPN) is a unique transmembrane receptor that promotes tumor cell motility. Indeed, PDPN may serve as a chemotherapeutic target for primary and metastatic cancer cells, particularly oral squamous cell carcinoma (OSCC) cells that cause most oral cancers. Here, we studied how a monoclonal antibody (NZ-1) and lectin (MASL) that target PDPN affect human OSCC cell motility and viability. Both reagents inhibited the migration of PDPN expressing OSCC cells at nanomolar concentrations before inhibiting cell viability at micromolar concentrations. In addition, both reagents induced mitochondrial membrane permeability transition to kill OSCC cells that express PDPN by caspase independent nonapoptotic necrosis. Furthermore, MASL displayed a surprisingly robust ability to target PDPN on OSCC cells within minutes of exposure, and significantly inhibited human OSCC dissemination in zebrafish embryos. Moreover, we report that human OSCC cells formed tumors that expressed PDPN in mice, and induced PDPN expression in infiltrating host murine cancer associated fibroblasts. Taken together, these data suggest that antibodies and lectins may be utilized to combat OSCC and other cancers that express PDPN.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.