miR-372 inhibits p62 in head and neck squamous cell carcinoma in vitro and in vivo
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Li-Yin Yeh1, Chung-Ji Liu2,3, Yong-Kie Wong2,4, Christine Chang1, Shu-Chun Lin1,2,5 and Kuo-Wei Chang1,2,5
1 Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan
2 Department of Dentistry, National Yang-Ming University, Taipei, Taiwan
3 Department of Dentistry, Taipei Mackay Memorial Hospital, Taipei, Taiwan
4 Department of Dentistry, Taichung Veterans General Hospital, Taichung, Taiwan
5 Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan
Shu-Chun Lin, email:
Kuo-Wei Chang, email:
Keywords: Carcinoma, Hypoxia, Invasion, miR-372, Mouth
Received: November 26, 2014 Accepted: January 05, 2015 Published: January 21, 2015
Here we showed that exogenous miR-372 expression and knockdown of p62 (sequestosome1 or SQSTM1), both increased migration of head and neck squamous cell carcinoma (HNSCC) cells. p62 induced phase II detoxification enzyme NADPH quinone oxidoreductase 1 (NQO1), which decreased ROS levels and cell migration. Also, miR-372 decreased p62 during hypoxia, thus increasing cell migration. Levels of miR-372 and p62 inversely correlated in human HNSCC tissues. Plasma levels of miR-372 was associated with advanced tumor stage and patient mortality. Both plasma and salivary miR-372 levels were decreased after tumor resection. We conclude that miR-372 decreases p62, thus increasing ROS and motility in HNSCC cells.
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