Fluorescent humanized anti-CEA antibody specifically labels metastatic pancreatic cancer in a patient-derived orthotopic xenograft (PDOX) mouse model
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Thinzar M. Lwin1, Kentaro Miyake1,2,3, Takashi Murakami1,2,3, Jonathan C. DeLong1, Siamak Amirfakhri4, Filemoni Filemoni4, Sang Nam Yoon1,2, Paul J. Yazaki5, John E. Shivley5, Brian Datnow6, Bryan M. Clary1, Robert M. Hoffman1,2,4 and Michael Bouvet1,4
1Department of Surgery, University of California San Diego, San Diego, CA, USA
2AntiCancer, Inc., San Diego, CA, USA
3Department of Gastroenterological Surgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan
4VA San Diego Healthcare System, San Diego, CA, USA
5Department of Molecular Imaging and Therapy, City of Hope, Duarte, CA, USA
6Department of Pathology, University of California San Diego, San Diego, CA, USA
Michael Bouvet, email: [email protected]
Keywords: fluorescence-guided surgery; LICOR IRDye800CW; anti-CEA antibody; patient derived orthotopic xenograft model; pancreatic cancer
Received: July 05, 2018 Accepted: December 04, 2018 Published: December 18, 2018
Pancreatic cancer is a highly lethal disease in part due to incomplete tumor resection. Targeting by tumor-specific antibodies conjugated with a fluorescent label can result in selective labeling of cancer in vivo for surgical navigation. In the present study, we describe a patient-derived orthotopic xenograft model of pancreatic cancer that recapitulated the disease on a gross and microscopic level, along with physiologic clinical manifestations. We additionally show that the use of an anti-CEA antibody conjugated to the near-infrared (NIR) fluorescent dye, IRDye800CW, can selectively highlight the pancreatic cancer and its metastases in this model with a tumor-to-background ratio of 3.5 (SEM 0.9). The present results demonstrate the clinical potential of this labeling technique for fluorescence-guided surgery of pancreatic cancer.
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