Research Papers:

Ribonuclease MCPiP1 contributes to the loss of micro-RNA-200 family members in pancreatic cancer cells

Françoise Boudouresque, Carole Siret, Aurélie Dobric, Françoise Silvy, Philippe Soubeyran, Juan Iovanna, Dominique Lombardo and Yolande Berthois _

PDF  |  HTML  |  Supplementary Files  |  How to cite

Oncotarget. 2018; 9:35941-35961. https://doi.org/10.18632/oncotarget.26310

Metrics: PDF 1166 views  |   HTML 1813 views  |   ?  


Françoise Boudouresque1, Carole Siret1,2, Aurélie Dobric1,3, Françoise Silvy1,3, Philippe Soubeyran3, Juan Iovanna3, Dominique Lombardo1 and Yolande Berthois1,3

1Aix-Marseille Univ, INSERM UMR 911, CRO2, Marseille, France

2Aix-Marseille Univ, CNRS, INSERM, CIML Marseille, France

3Present address: Aix-Marseille University, INSERM, CNRS, Institut Paoli-Calmettes, CRCM, Pancreatic Cancer Team, Marseille, France

Correspondence to:

Yolande Berthois, email: [email protected]

Keywords: pancreatic cancer; micro-RNA-200; MCPiP1; dicer1

Received: April 04, 2018     Accepted: October 25, 2018     Published: November 13, 2018


The microRNA-200 (miR-200) family is frequently down-regulated in tumors, including pancreatic adenocarcinomas (PDACs). In this study we have examined the mechanisms involved in the loss of miR-200s in tumoral pancreatic cells. Whereas miR-200 gene promoters appear methylated in mature miR-200 deficient cell lines, miR-200 precursors are detected in nuclear but not cytoplasmic compartment of these cells, indicating that promoter hypermethylation is not sufficient to explain the deficit of mature miR-200s. The ribonuclease Monocyte Chemotactic Protein-induced Protein-1 (MCPiP1) may counteract Dicer1 in miRNA maturation process. MCPiP1/Dicer1 mRNA and protein ratios appear higher in miR-200 deficient compared to miR-200 proficient cells, suggesting that MCPiP1 may compete with Dicer1 in mature miR-200 deficient cells. Inhibition of MCPiP1 allows the detection of miR-200 precursors in cytoplasm of miR-200 deficient cells, confirming its involvement in the loss of miR-200s. Also, reversion of MCPiP1/Dicer1 ratio by over-expression of Dicer1 in miR-200 deficient cells leads to the recovery of mature miR-200s. Finally, whereas human malignant pancreatic tissues (PDACs) express lower miR-200 levels than non malignant tissues (non-MPDs), MCPiP1/Dicer1 ratio appears higher in PDACs, when compared to non-MPDs, supporting the hypothesis that MCPiP1/Dicer1 ratio is determinant in regulating miR-200 maturation process in a subset of tumoral pancreatic cells.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 26310