Oncotarget

Research Papers:

CRISPR/Cas9 nickase mediated targeting of urokinase receptor gene inhibits neuroblastoma cell proliferation

Karina D. Rysenkova, Ekaterina V. Semina _, Maxim N. Karagyaur, Anna A. Shmakova, Daniyar T. Dyikanov, Petr A. Vasiluev, Yury P. Rubtsov, Kseniya A. Rubina and Vsevolod A. Tkachuk

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Oncotarget. 2018; 9:29414-29430. https://doi.org/10.18632/oncotarget.25647

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Abstract

Karina D. Rysenkova1, Ekaterina V. Semina1,2, Maxim N. Karagyaur3, Anna A. Shmakova1, Daniyar T. Dyikanov1, Petr A. Vasiluev1, Yury P. Rubtsov1,4, Kseniya A. Rubina1 and Vsevolod A. Tkachuk1,2

1Lomonosov Moscow State University, Faculty of Medicine, Laboratory of Gene and Cell Technologies, 119991, Moscow, Russian Federation

2Federal State Budgetary Organization National Cardiology Research Center Ministry of Health of the Russian Federation, Institute of Experimental Cardiology, 121552, Moscow, Russian Federation

3Institute of Regenerative Medicine, Lomonosov Moscow State University, 119991, Moscow, Russian Federation

4Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997, Moscow, Russian Federation

Correspondence to:

Ekaterina V. Semina, email: e-semina@yandex.ru

Keywords: CRISPR/Cas9; genome editing; urokinase receptor; cell proliferation; neuroblastoma

Received: January 29, 2018     Accepted: June 05, 2018     Published: June 29, 2018

ABSTRACT

Neuroblastoma is a tumor arising from pluripotent sympathoadrenal precursor cells of neural cell origin. Neuroblastoma is one of the most aggressive childhood tumors with highly invasive and metastatic potential. The increased expression of urokinase and its receptor is often associated with a negative prognosis in neuroblastoma patients.

We have shown that targeting of the Plaur gene in mouse neuroblastoma Neuro 2A cells by CRISPR/Cas9n results in ~60% decrease in cell proliferation (p<0.05), reduction in the number of Ki-67 positive cells, caspase 3 activation and PARP-1 cleavage. Knockout of uPAR leads to downregulation of mRNA encoding full-length TrkC receptor, which is involved in p38MAPK and Akt signalling pathways. This finding provides a rationale to study a role of uPAR in neuroblastoma progression, since uPAR could be considered a potential therapeutic target in neuroblastoma treatment.


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