Oncotarget

Clinical Research Papers:

The fallopian tube microbiome: implications for reproductive health

Elise S. Pelzer _, Dana Willner, Melissa Buttini, Louise M. Hafner, Christina Theodoropoulos and Flavia Huygens

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Oncotarget. 2018; 9:21541-21551. https://doi.org/10.18632/oncotarget.25059

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Abstract

Elise S. Pelzer1,2, Dana Willner3, Melissa Buttini4, Louise M. Hafner1, Christina Theodoropoulos1 and Flavia Huygens1

1Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Queensland University of Technology, Brisbane, Queensland, 4001 Australia

2The Wesley Research Institute, Women’s Health Laboratory, The Wesley Hospital, Auchenflower, Queensland, 4066 Australia

3The Australian Centre for Ecogenomics, St Lucia, Queensland, 4067 Australia

4The Wesley Hospital, Auchenflower, Queensland, 4066 Australia

Correspondence to:

Elise S. Pelzer, email: e.pelzer@qut.edu.au

Keywords: fallopian tube; microbiome; menstrual cycle

Received: July 13, 2017     Accepted: March 21, 2018     Published: April 20, 2018

ABSTRACT

Objective: There is a paucity of data characterizing the microbiota of the female upper genital tract, which controversially is described as a sterile site. We examine whether the fallopian tube harbours an endogenous microbial community.

Design: This prospective study collected from women undergoing total hysterectomy or salpingectomy-oophorectomy.

Setting: Private hospital gynaecology department.

Patients: Fallopian tubes were collected from women diagnosed with benign disease or for prophylaxis.

Interventions: Samples were interrogated for the presence of microbial DNA using a next generation sequencing technology approach to exploit the V5 to V9 regions of the 16S rRNA gene.

Main outcome measures: The fallopian tube microbiota was characterized using traditional culture techniques and next generation sequencing.

Results: Bacteria were isolated from 50% of cultured samples, and 100% of samples returned positive PCR results. Only 68% of the culture isolates could be confidently identified using automated diagnostic equipment in a clinical microbiology laboratory. Monomicrobial communities were identified only for cultured isolates (50%). Pyrosequencing revealed that all communities were polymicrobial. Lactobacillus spp. were not present in all groups, nor were they the most dominant isolates. Distinct differences in the microbial communities were evident for left compared to right fallopian tubes, ampulla versus isthmus, pre- and post- menopausal tissue, and in secretory phase fallopian tubes with and without Mirena intrauterine devices in situ (all p < 0.05).

Conclusion: The female upper genital tract is not sterile. Distinct microbial community profiles in the fallopian tubes of healthy women suggest that this genital tract site supports an endogenous microbiota.


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