Oncotarget

Research Papers:

Development of a reliable assay to measure glypican-1 in plasma and serum reveals circulating glypican-1 as a novel prostate cancer biomarker

Rachel A. Levin, Maria E. Lund, Quach Truong, Angela Wu, Neal D. Shore, Daniel R. Saltzstein, Raoul S. Concepcion, Thomas A. Paivanas, Arletta van Breda, Jennifer Beebe-Dimmer, Julie J. Ruterbusch, Sandra Wissmueller, Douglas H. Campbell and Bradley J. Walsh _

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Oncotarget. 2018; 9:22359-22367. https://doi.org/10.18632/oncotarget.25009

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Abstract

Rachel A. Levin1, Maria E. Lund1, Quach Truong1, Angela Wu1, Neal D. Shore2, Daniel R. Saltzstein2, Raoul S. Concepcion2, Thomas A. Paivanas2, Arletta van Breda2, Jennifer Beebe-Dimmer3, Julie J. Ruterbusch3, Sandra Wissmueller1, Douglas H. Campbell1 and Bradley J. Walsh1

1Minomic International Ltd, Sydney, New South Wales, Australia

2CUSP LLC Research Consortium, Annandale, VA, USA

3Barbara Ann Karmanos Cancer Institute and Wayne State University, School of Medicine, Department of Oncology, Detroit, MI, USA

Correspondence to:

Bradley J. Walsh, email: brad.walsh@minomic.com

Keywords: prostate cancer; biomarker; glypican-1; MIL-38; 3G5

Received: October 07, 2017     Accepted: March 11, 2018     Published: April 27, 2018

ABSTRACT

Prostate cancer is responsible for hundreds of thousands of annual deaths worldwide. The current gold standard in early detection of prostate cancer, the prostate specific antigen test, boasts a high sensitivity but low specificity, resulting in many unnecessary prostate biopsies. Thus, emphasis has been placed on identifying new biomarkers to improve prostate cancer detection. Glypican-1 has recently been proposed as one such biomarker, however further exploration into its predictive power has been hindered by a lack of available, dependable glypican-1 immunoassays. Previously, we identified human glypican-1 as the antigenic target of the MIL-38 monoclonal antibody. Additionally, we have now generated another monoclonal antibody, 3G5, that also recognizes human glypican-1. Here we report the development of a reliable, custom Luminex® assay that enables precise quantitation of circulating human glypican-1 in plasma and serum. Using this assay, we show for the first time that circulating glypican-1 levels can differentiate non-cancer (normal and benign prostatic hyperplasia) patients from prostate cancer patients, as well as benign prostatic hyperplasia patients alone from prostate cancer patients. Our findings strongly promote future investigation into the use of glypican-1 for early detection of prostate cancer.


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