Comprehensive analysis of telomerase inhibition by gallotannin
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Nikita Savelyev1, Polina Baykuzina2, Svetlana Dokudovskaya3, Olga Lavrik4,5, Maria Rubtsova1,6 and Olga Dontsova1,6
1Lomonosov Moscow State University, Department of Chemistry, and A.N. Belozersky Institute of Physico-Chemical Biology, Moscow, 119992, Russia
2Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow, 119992, Russia
3UMR 8126, CNRS, University Paris-Sud, Université Paris Saclay, Institut de Cancérologie Gustave-Roussy, 94805, Villejuif, France
4Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia
5Novosibirsk State University, Novosibirsk, 630090, Russia
6Skolkovo Institute of Science and Technology, Skolkovo, Moscow region, 143025, Russia
Maria Rubtsova, email: [email protected]
Keywords: telomerase; telomere; gallotannin; inhibition; poly(ADP-ribosy)lation
Received: August 18, 2017 Accepted: February 24, 2018 Published: April 10, 2018
Gallotannin (or tannic acid) is a naturally occurring compound that inhibits cell growth and activity of different DNA-polymerases, including telomerase. The purpose of the present study was to gain insight into the mechanism of telomerase inhibition by gallotannin. We determined that gallotannin inhibits telomerase in vitro with an half maximal inhibitory concentrations value of 130 nM, but it does not affect telomerase complex assembly and component levels in vivo. The inhibitory activity of gallotannin against telomerase provides an additional explanation for the anti-cancer activities of this compound.
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