Oncotarget

Research Papers:

Transcriptional orchestration of mitochondrial homeostasis in a cellular model of PGC-1-related coactivator-dependent thyroid tumor

Solenne Dumont, Soazig Le Pennec, Audrey Donnart, Raluca Teusan, Marja Steenman, Catherine Chevalier, Rémi Houlgatte and Frédérique Savagner _

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Oncotarget. 2018; 9:15883-15894. https://doi.org/10.18632/oncotarget.24633

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Abstract

Solenne Dumont1,*, Soazig Le Pennec2,*, Audrey Donnart1, Raluca Teusan1, Marja Steenman1, Catherine Chevalier3, Rémi Houlgatte4 and Frédérique Savagner2

1L’institut du Thorax, INSERM, CNRS, UNIV Nantes, BP 70721, 44007 NANTES Cedex 1, France

2Inserm UMR 1048, I2MC, 31432 TOULOUSE Cedex 4, France

3UMS 016, SFR Santé, IRS UN, BP 70721, 44007 NANTES Cedex 1, France

4Inserm UMR 954, Faculté de Médecine, BP 184, 54505 VANDOEUVRE-LÈS-NANCY Cedex, France

*These authors have contributed equally to this work

Correspondence to:

Frédérique Savagner, email: Frederique.savagner@inserm.fr

Keywords: PGC-1-related coactivator; transcription factors; miRNAs; integrative network

Received: November 08, 2017     Accepted: February 26, 2018     Published: March 23, 2018

ABSTRACT

The PGC-1 (Peroxisome proliferator-activated receptor Gamma Coactivator-1) family of coactivators (PGC-1α, PGC-1β, and PRC) plays a central role in the transcriptional control of mitochondrial biogenesis and oxidative phosphorylation (OXPHOS) processes. These coactivators integrate mitochondrial energy production into cell metabolism using complementary pathways. The XTC.UC1 cell line is a mitochondria-rich model of thyroid tumors whose biogenesis is almost exclusively dependent on PRC. Here we aim to propose an integrative view of the cellular pathways regulated by PRC through integration of cDNA and miRNA microarray data and chromatin immunoprecipitation results obtained from XTC.UC1 cells invalidated for PRC. This study showes that PRC induces a complex network of cellular functions interacting with at least one to five of the studied transcription factors (Estrogen Related Receptor alpha, ERR1; Nuclear-Respiratory Factors, NRF1 and NRF2; cAMP Response Element Binding, CREB; and Ying Yang, YY1). Our data confirm that ERR1 is a key partner of PRC in the regulation of mitochondrial functions and suggest a potential role of this complex in RNA processing. PRC is also involved in transcriptional regulatory complexes targeting 12 miRNAs, five of which are involved in the control of the OXPHOS process. Our findings demonstrate that the PRC coactivator can act in complex with several transcription factors and regulate miRNA expression to control the fine regulation of main metabolic functions in the cell. Therefore, in PGC-1α/β-associated pathologies, PRC, as a metabolic sensor, may ensure mitochondrial homeostasis.


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