The lncRNA H19 positively affects the tumorigenic properties of glioblastoma cells and contributes to NKD1 repression through the recruitment of EZH2 on its promoter
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Barbara Fazi1, Sabrina Garbo1, Nicola Toschi1,2,3, Annunziato Mangiola4, Malinska Lombari1, Daria Sicari1,7, Cecilia Battistelli5, Silvia Galardi1, Alessandro Michienzi1, Gianluca Trevisi4, Rona Harari-Steinfeld6, Carla Cicchini5 and Silvia Anna Ciafrè1
1Department of Biomedicine and Prevention, University of Rome “Tor Vergata”, Rome, Italy
2Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Boston, MA, USA
3Harvard Medical School, Boston, MA, USA
4Department Head and Neck, Institute of Neurosurgery, Catholic University of Sacred Heart, Rome, Italy
5Department of Cellular Biotechnologies and Haematology, Sezione di Genetica Molecolare, Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Sapienza University of Rome, Rome, Italy
6Goldyne Savad Institute of Gene Therapy, Hadassah University Hospital, Hebrew University, Jerusalem
7Present address: Laboratorio Nazionale CIB (LNCIB), AREA Science Park, Trieste, Italy
Silvia Anna Ciafrè, email: [email protected]
Keywords: glioblastoma; lncRNA; H19; NKD1; EZH2
Received: July 21, 2017 Accepted: February 10, 2018 Epub: February 14, 2018 Published: March 20, 2018
The still largely obscure molecular events in the glioblastoma oncogenesis, a primary brain tumor characterized by an inevitably dismal prognosis, impel for investigation. The importance of Long noncoding RNAs as regulators of gene expression has recently become evident. Among them, H19 has a recognized oncogenic role in several types of human tumors and was shown to correlate to some oncogenic aspects of glioblastoma cells. Here we, hypothesyze that in glioblastoma H19 exerts its function through the interaction with the catalytic subunit of the PRC2 complex, EZH2. By employing a factor analysis on a SAGE dataset of 12 glioblastoma samples, we show that H19 expression in glioblastoma tissues correlates with that of several genes involved in glioblastoma growth and progression. H19 knock-down reduces viability, migration and invasiveness of two distinct human glioblastoma cell lines. Most importantly, we provide a mechanistic perspective about the role of H19 in glioblastoma cells, by showing that its expression is inversely linked to that of NKD1, a negative regulator of Wnt pathway, suggesting that H19 might regulate NKD1 transcription via EZH2-induced H3K27 trimethylation of its promoter. Indeed, we showed that H19 binds EZH2 in glioblastoma cells, and that EZH2 binding to NKD1 and other promoters is impaired by H19 silencing.
In this work we describe H19 as part of an epigenetic modulation program executed by EZH2, that results in the repression of Nkd1. We believe that our results can provide a new piece to the complex puzzle of H19 function in glioblastoma.
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