Quantitative mass spectrometry analysis reveals a panel of nine proteins as diagnostic markers for colon adenocarcinomas
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Apurva Atak1, Samiksha Khurana1, Kishore Gollapalli1, Panga Jaipal Reddy1, Roei Levy2, Stav Ben-Salmon2, Dror Hollander2, Maya Donyo2, Anke Heit3, Agnes Hotz-Wagenblatt3, Hadas Biran4, Roded Sharan4, Shailendra Rane5, Ashutosh Shelar5, Gil Ast2 and Sanjeeva Srivastava1
1Proteomics Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
2Department of Human Molecular Genetics and Biochemistry, Sackler Medical School, Tel Aviv University, Tel Aviv 69978, Israel
3Bioinformatics Group, Genomics and Proteomics Core Facility (GPCF), German Cancer Research Center (DKFZ), Heidelberg 69120, Germany
4Blavatnik School of Computer Science, Tel Aviv University, Tel Aviv 69978, Israel
5Shimadzu Analytical (India) Pvt. Ltd, 1A/B, Rushabh Chambers, Makwana Road, Marol, Andheri (E), Mumbai 400059, India
Sanjeeva Srivastava, email: [email protected]
Keywords: colon adenocarcinoma; proteomics; biomarkers; tissue; iTRAQ
Received: July 14, 2017 Accepted: January 30, 2018 Published: February 05, 2018
Adenocarcinomas are cancers originating from the gland forming cells of the colon and rectal lining, and are known to be the most common type of colorectal cancers. The current diagnosis strategies for colorectal cancers include biopsy, laboratory tests, and colonoscopy which are time consuming. Identification of protein biomarkers could aid in the detection of colon adenocarcinomas (CACs). In this study, tissue proteome of colon adenocarcinomas (n = 11) was compared with the matched control specimens (n = 11) using isobaric tags for relative and absolute quantitation (iTRAQ) based liquid chromatography-mass spectrometry (LC-MS/MS) approach. A list of 285 significantly altered proteins was identified in colon adenocarcinomas as compared to its matched controls, which are associated with growth and malignancy of the tumors. Protein interaction analysis revealed the association of altered proteins in colon adenocarcinomas with various transcription factors and their targets. A panel of nine proteins was validated using multiple reaction monitoring (MRM). Additionally, S100A9 was also validated using immunoblotting. The identified panel of proteins may serve as potential biomarkers and thereby aid in the detection of colon adenocarcinomas.
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