Oncotarget

Research Papers:

Linc00441 interacts with DNMT1 to regulate RB1 gene methylation and expression in gastric cancer

Jianping Zhou _, Jun Shi, Xingli Fu, Boneng Mao, Weimin Wang, Weiling Li, Gang Li and Sujun Zhou

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Oncotarget. 2018; 9:37471-37479. https://doi.org/10.18632/oncotarget.23928

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Abstract

Jianping Zhou1,*, Jun Shi1,*, Xingli Fu2,*, Boneng Mao3, Weimin Wang4, Weiling Li5, Gang Li5 and Sujun Zhou1

1Department of General Surgery, Yixing People’s Hospital The Affiliated Hospital of Jiangsu University, Yixing 214200, Jiangsu, P.R China

2Health Science Center, Jiangsu University, Zhenjiang 212000, Jiangsu, P.R China

3Department of Gastroenterology, Yixing People’s Hospital The Affiliated Hospital of Jiangsu University, Yixing 214200, Jiangsu, P.R China

4Department of Oncology, Yixing People’s Hospital The Affiliated Hospital of Jiangsu University, Yixing 214200, Jiangsu, P.R China

5Yixing People’s Hospital The Affiliated Hospital of Jiangsu University, Yixing 214200, Jiangsu, P.R China

*These authors contributed equally to this work

Correspondence to:

Sujun Zhou, email: staff030@sina.com

Jianping Zhou, email: staff882@sina.com

Keywords: gastric cancer; linc00441; RB1; DNMT1; proliferation

Received: August 05, 2017     Accepted: November 03, 2017     Epub: January 03, 2018     Published: December 25, 2018

ABSTRACT

Recent studies revealed that several Long noncoding RNAs (LncRNAs) are associated with progression of gastric cancer (GC), while the functional role and molecular mechanism of many GC-associated lncRNAs remain undetermined. The tumor suppressor-gene retinoblastoma gene (RB1) was decreased in several human cancers including gastric cancer (GC). In this study, we investigated whether Linc00441 was involved in the suppression of RB1. Our findings showed that the up-regulated Linc00441 was inversely correlated with RB1 expression in human GC tumor samples. The gain- and loss-of-function investigation revealed that Linc00441 could promote the proliferation of GC cells. Furthermore, RNA pull down and RIP assays demonstrated that Linc00441 could recruit DNMT1 to the RB1 promoter and suppressed RB1 expression in GC cells. In conclusion, our findings revealed that Linc00441 played crucial role in GC progression and suggested that Linc00441 was potentially an effective target for GC therapy in the future.


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