Oncotarget

Research Papers:

Identification and functional characterization of circRNA-0008717 as an oncogene in osteosarcoma through sponging miR-203

Xiang Zhou, Dimple Natino, Zili Qin, Dong Wang, Zhen Tian, Xuan Cai, Bo Wang and Xijing He _

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Oncotarget. 2018; 9:22288-22300. https://doi.org/10.18632/oncotarget.23466

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Abstract

Xiang Zhou1, Dimple Natino2, Zili Qin3, Dong Wang1, Zhen Tian4, Xuan Cai1, Bo Wang5 and Xijing He1

1Department of Orthopaedics, Second Affiliated Hospital of Xi’an Jiaotong University, Xi'an, China

2Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL, USA

3Department of Otolaryngology, Otorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China

4Department of Infectious Diseases, First Affiliated Hospital of Xi’an Jiaotong University, Xi'an, China

5Department of Pharmacology, School of Basic Medical Science, Xi’an Jiaotong University Health Science Center, Xi'an, China

Correspondence to:

Xijing He, email: xijing_h@vip.tom.com

Keywords: osteosarcoma; circRNA-0008717; miR-203; Bmi-1

Received: September 11, 2017     Accepted: November 16, 2017     Epub: December 20, 2017     Published: April 27, 2018

ABSTRACT

Circular RNA (circRNA) is a key regulator in the development and progression of human cancers, however its role in osteosarcoma tumorigenesis is not well understood. The present study aims to investigate the expression profiles and potential modulation of circRNA on osteosarcoma carcinogenesis. Human circRNA microarray was performed to screen for abnormally expressed circRNA in osteosarcoma tissue and circRNA-0008717 was identified as one circRNA significantly upregulated in osteosarcoma tissue. Osteosarcoma patients with high circRNA-0008717 expression had shortened survival. Gain and loss functional assays suggested that knockdown of circRNA-0008717 suppressed cell proliferation, migration and invasion, but promoted cell apoptosis. By using biotin-labeled circRNA-0008717 probe to perform RNA precipitation in osteosarcoma cells, we identified miR-203 as the circ0008717-associated microRNA. Subsequently, Bmi-1 was identified as the functional target of miR-203. In addition, overexpression of circRNA-0008717 in osteosarcoma could elevate Bmi-1 expression, resulting in the promotion of osteosarcoma cell proliferation and invasion. Furthermore, the tumor promoting effect of circRNA-0008717 was abolished by miR-203 mimics or Bmi-1 silencing vector. In conclusion, circRNA-0008717 plays an oncogenic role in osteosarcoma and may serve as a promising prognostic biomarker for osteosarcoma patients. Therefore, silence of circRNA-0008717 could be a future direction to develop a novel treatment strategy.


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