Research Papers:

Utilizing glycine N-methyltransferasegene knockout mice as a model for identification of missing proteins in hepatocellular carcinoma

Ming-Hui Yang, Wan-Jou Chen, Yaw-Syan Fu, Bin Huang, Wan-Chi Tsai, Yi-Ming Arthur Chen, Po-Chiao Lin, Cheng-Hui Yuan and Yu-Chang Tyan _

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Oncotarget. 2018; 9:442-452. https://doi.org/10.18632/oncotarget.23064

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Ming-Hui Yang1, Wan-Jou Chen2, Yaw-Syan Fu1,3, Bin Huang1,3, Wan-Chi Tsai4, Yi-Ming Arthur Chen1,5, Po-Chiao Lin6, Cheng-Hui Yuan7 and Yu-Chang Tyan1,2,5,8,9

1Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung, Taiwan

2Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan

3Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan

4Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan

5Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

6Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan

7Mass Spectrometry Laboratory, Department of Chemistry, National University of Singapore, Singapore

8Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, Taiwan

9Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

Correspondence to:

Yu-Chang Tyan, email: [email protected]

Keywords: glycine N-methyltransferase; hepatocellular carcinoma; missing protein; proteomics; human proteome atlas

Received: August 01, 2017     Accepted: November 13, 2017     Published: December 07, 2017


Glycine N-methyltransferase is a tumor suppressor gene for hepatocellular carcinoma, which can activate DNA methylation by inducing the S-adenosylmethionine to S-adenosylhomocystine. Previous studies have indicated that the expression of Glycine N-methyltransferase is inhibited in hepatocellular carcinoma. To confirm and identify missing proteins, the pathologic analysis of the tumor-bearing mice will provide critical histologic information. Such a mouse model is applied as a screening tool for hepatocellular carcinoma as well as a strategy for missing protein discovery. In this study we designed an analysis platform using the human proteome atlas to compare the possible missing proteins to human whole chromosomes. This will integrate the information from animal studies to establish an optimal technique in the missing protein biomarker discovery.

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