Genome-wide analysis of the human malaria parasite Plasmodium falciparum transcription factor PfNF-YB shows interaction with a CCAAT motif
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Wânia Rezende Lima1,*, David Correa Martins2, Kleber Simônio Parreira3,*, Pedro Scarpelli1, Miriam Santos de Moraes1, Pantelis Topalis4, Ronaldo Fumio Hashimoto5 and Célia R.S. Garcia1
1Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil
2Centro de Matemática, Computação e Cognição, Universidade Federal do ABC, Santo André, Brazil
3Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil
4Institute of Molecular Biology and Biotechnology, FORTH, Hellas, Greece
5Departamento de Ciência da Computação, Instituto de Matemática e Estatística, Universidade de São Paulo, São Paulo, Brazil
*Instituto de Ciências Exatas e Naturais-Medicina, Universidade Federal de Mato Grosso-Campus Rondonópolis, Mato Grosso, Brazil
Célia R.S. Garcia, email: email@example.com
Keywords: transcription factor; CCAAT-box; malaria; Plasmodium falciparum; signaling
Received: March 24, 2017 Accepted: November 26, 2017 Published: December 09, 2017
Little is known about transcription factor regulation during the Plasmodium falciparum intraerythrocytic cycle. In order to elucidate the role of the P. falciparum (Pf)NF-YB transcription factor we searched for target genes in the entire genome. PfNF-YB mRNA is highly expressed in late trophozoite and schizont stages relative to the ring stage. In order to determine the candidate genes bound by PfNF-YB a ChIP-on-chip assay was carried out and 297 genes were identified. Ninety nine percent of PfNF-YB binding was to putative promoter regions of protein coding genes of which only 16% comprise proteins of known function. Interestingly, our data reveal that PfNF-YB binding is not exclusively to a canonical CCAAT box motif. PfNF-YB binds to genes coding for proteins implicated in a range of different biological functions, such as replication protein A large subunit (DNA replication), hypoxanthine phosphoribosyltransferase (nucleic acid metabolism) and multidrug resistance protein 2 (intracellular transport).
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