Research Papers:

Genome-wide analysis of the human malaria parasite Plasmodium falciparum transcription factor PfNF-YB shows interaction with a CCAAT motif

Wânia Rezende Lima, David Correa Martins, Kleber Simônio Parreira, Pedro Scarpelli, Miriam Santos de Moraes, Pantelis Topalis, Ronaldo Fumio Hashimoto and Célia R.S. Garcia _

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Oncotarget. 2017; 8:113987-114001. https://doi.org/10.18632/oncotarget.23053

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Wânia Rezende Lima1,*, David Correa Martins2, Kleber Simônio Parreira3,*, Pedro Scarpelli1, Miriam Santos de Moraes1, Pantelis Topalis4, Ronaldo Fumio Hashimoto5 and Célia R.S. Garcia1

1Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil

2Centro de Matemática, Computação e Cognição, Universidade Federal do ABC, Santo André, Brazil

3Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil

4Institute of Molecular Biology and Biotechnology, FORTH, Hellas, Greece

5Departamento de Ciência da Computação, Instituto de Matemática e Estatística, Universidade de São Paulo, São Paulo, Brazil

*Instituto de Ciências Exatas e Naturais-Medicina, Universidade Federal de Mato Grosso-Campus Rondonópolis, Mato Grosso, Brazil

Correspondence to:

Célia R.S. Garcia, email: cgarcia@usp.br

Keywords: transcription factor; CCAAT-box; malaria; Plasmodium falciparum; signaling

Received: March 24, 2017    Accepted: November 26, 2017    Published: December 09, 2017


Little is known about transcription factor regulation during the Plasmodium falciparum intraerythrocytic cycle. In order to elucidate the role of the P. falciparum (Pf)NF-YB transcription factor we searched for target genes in the entire genome. PfNF-YB mRNA is highly expressed in late trophozoite and schizont stages relative to the ring stage. In order to determine the candidate genes bound by PfNF-YB a ChIP-on-chip assay was carried out and 297 genes were identified. Ninety nine percent of PfNF-YB binding was to putative promoter regions of protein coding genes of which only 16% comprise proteins of known function. Interestingly, our data reveal that PfNF-YB binding is not exclusively to a canonical CCAAT box motif. PfNF-YB binds to genes coding for proteins implicated in a range of different biological functions, such as replication protein A large subunit (DNA replication), hypoxanthine phosphoribosyltransferase (nucleic acid metabolism) and multidrug resistance protein 2 (intracellular transport).

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