Oncotarget

Research Papers:

Integrated hepatic transcriptional and serum metabolic studies on circulating nutrient metabolism in diurnal laying hens

Wan Dan, Liu Yi-Lin, Li Guan-Ya, Huang Rui-Lin, Zhang Yi-Ming, Long Ci-Min, Ruan Zheng, Li Lan, Wu Xin _, Zhou Xi-Hong and Yin Yu-Long

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Oncotarget. 2017; 8:113885-113894. https://doi.org/10.18632/oncotarget.23032

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Abstract

Wan Dan1,2, Liu Yi-Lin1,2, Li Guan-Ya1, Huang Rui-Lin1, Zhang Yi-Ming1,3, Long Ci-Min1, Ruan Zheng2, Li Lan1, Wu Xin1,2, Zhou Xi-Hong1 and Yin Yu-Long1,2,3

1Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Science, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Engineering Research Center of Healthy Livestock, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, China

2School of Food Science and Technology, State Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China

3Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan Normal University, Changsha, Hunan 410125, China

Correspondence to:

Wu Xin, email: wuxin@isa.ac.cn

Zhou Xi-Hong, email: xhzhou@isa.ac.cn

Yin Yu-Long, email: yinyulong@isa.ac.cn

Keywords: circadian rhythm; laying hens; hepatic transcriptome; circulating nutrients

Received: July 26, 2017     Accepted: October 30, 2017     Published: December 07, 2017

ABSTRACT

The aim of the study was to see the diurnal variation of nutrients metabolism and their regulation under the management of large-scaled production. The hepatic transcriptional and serum metabolic studies on circulating nutrient metabolism were investigated in diurnal laying hens. Liver and blood were collected from 36 hens that were slaughtered at 3:30, 7:30, 11:30, 15:30, 19:30, and 23:30 (n = 6), respectively. The serum amino acid, fatty acid and glucose levels, as well as the hepatic transcriptome were analyzed. The results revealed that the circadian clock genes such as Bmal1, Clock, Per1, and Cry2 displayed circadian rhythms in hen livers. The genes related to circulating nutrient transportation, lipogenesis, lipid catabolism, sterol metabolism, and oxidative/anti-oxidative systems also oscillated. However, the nadir of glucose was observed at 7:30 and peaked at 11:30 in the day. Amino acid levels peaked mainly at night, and most amino acids exhibited circadian rhythms based on CircWave analysis. With the exception of undecanoic acid (C11:0), myristoleic acid (C14:1), cis-11, 14-eicosenoic acid (C20:2), and (cis-4, 7, 10, 13, 16, 19-docosahexaenoic acid) C20:3N6 fatty acids, others peaked at 7:30 and 15:30. The results indicated that the hens required more glucose in the early morning. More proteins should be ingested late in the day, since protein catabolism occurred mostly at night. To remove the redundant fats and lipids, fewer should be ingested, especially during the night. All these results would help to design a more accurate nutrition schedule for improving the performance of laying hens in the future.


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