Oncotarget

Research Papers:

RNA-seq expression profiling of rat MCAO model following reperfusion Orexin-A

Chun-Mei Wang, Yan-You Pan, Ming-Hui Liu, Bao-Hua Cheng, Bo Bai _ and Jing Chen

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Oncotarget. 2017; 8:113066-113081. https://doi.org/10.18632/oncotarget.22995

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Abstract

Chun-Mei Wang1, Yan-You Pan1, Ming-Hui Liu1, Bao-Hua Cheng1, Bo Bai1 and Jing Chen1,2

1Neurobiology Institute, Jining Medical University, Jining, P.R. China

2Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, UK

Correspondence to:

Bo Bai, email: bobai@mail.jnmc.edu.cn

Jing Chen, email: Jing.Chen@warwick.ac.uk

Keywords: Orexin-A; ischemia-reperfusion injury; RNA sequencing; differential gene expression; gene network

Received: July 08, 2017    Accepted: August 27, 2017    Published: December 06, 2017

ABSTRACT

Orexin-A is a neuropeptide with potent neuroprotective activity towards cerebral ischemia-reperfusion (I/R) injury, but few studies have attempted to elucidate the mechanism. Herein, we performed global gene expression profiling of the hippocampus following reperfusion with Orexin-A using RNA sequencing (RNA-seq). RNA-seq identified 649 differentially expressed genes (DEGs) in the Orexin-A group compared with saline controls (I/R group), of which 149 were up-regulated and 500 were down-regulated. DEGs were confirmed using qRT-PCR, their molecular functions, biological processes and molecular components were explored using Gene Ontology (GO) analysis and 206 KEGG pathways were associated with Orexin-A treatment. MAPK, chemokine and calcium signalling pathways were mainly responsible for the neuroprotective effects of Orexin-A. Hspb1, Igf2 and Ptk2b were selected for functional interaction analysis by GeneMANIA. The results suggest that Orexin-A modifies gene expression in the hippocampus, leading to neuroprotection from I/R injury. The study provides a basis for future elucidation of the molecular mechanisms underlying Orexin-A.


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