Research Papers:
RNA-seq expression profiling of rat MCAO model following reperfusion Orexin-A
PDF | HTML | How to cite
Metrics: PDF 2189 views | HTML 3714 views | ?
Abstract
Chun-Mei Wang1, Yan-You Pan1, Ming-Hui Liu1, Bao-Hua Cheng1, Bo Bai1 and Jing Chen1,2
1Neurobiology Institute, Jining Medical University, Jining, P.R. China
2Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, UK
Correspondence to:
Bo Bai, email: [email protected]
Jing Chen, email: [email protected]
Keywords: Orexin-A; ischemia-reperfusion injury; RNA sequencing; differential gene expression; gene network
Received: July 08, 2017 Accepted: August 27, 2017 Published: December 06, 2017
ABSTRACT
Orexin-A is a neuropeptide with potent neuroprotective activity towards cerebral ischemia-reperfusion (I/R) injury, but few studies have attempted to elucidate the mechanism. Herein, we performed global gene expression profiling of the hippocampus following reperfusion with Orexin-A using RNA sequencing (RNA-seq). RNA-seq identified 649 differentially expressed genes (DEGs) in the Orexin-A group compared with saline controls (I/R group), of which 149 were up-regulated and 500 were down-regulated. DEGs were confirmed using qRT-PCR, their molecular functions, biological processes and molecular components were explored using Gene Ontology (GO) analysis and 206 KEGG pathways were associated with Orexin-A treatment. MAPK, chemokine and calcium signalling pathways were mainly responsible for the neuroprotective effects of Orexin-A. Hspb1, Igf2 and Ptk2b were selected for functional interaction analysis by GeneMANIA. The results suggest that Orexin-A modifies gene expression in the hippocampus, leading to neuroprotection from I/R injury. The study provides a basis for future elucidation of the molecular mechanisms underlying Orexin-A.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 22995