PCPA protects against monocrotaline-induced pulmonary arterial remodeling in rats: potential roles of connective tissue growth factor
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Yang Bai1, Zhong-Xia Li2, Yue-Tong Zhao1, Mo Liu1, Yun Wang1, Guo-Chao Lian1, Qi Zhao3,4 and Huai-Liang Wang1,5,6
1Department of Clinical Pharmacology, College of Pharmacy, China Medical University, Shenyang, 110122, China
2Department of Orthopaedic Ward, Central Hospital Affiliated Shenyang Medical College, Shenyang, 110122, China
3School of Mathematics, Liaoning University, Shenyang, 110036, China
4Research Center for Computer Simulating and Information Processing of Bio-Macromolecules of Liaoning Province, Shenyang, 110036, China
5National Key Subject, Institute of Respiratory Diseases, China Medical University, Shenyang, 110122, China
6Institute of Cardiovascular Diseases, China Medical University, Shenyang, 110122, China
Huai-Liang Wang, email: firstname.lastname@example.org
Qi Zhao, email: zhaoqi@Inu.edu.cn
Keywords: CTGF; PCPA; pulmonary arterial hypertension; monocrotaline; remodeling
Received: August 31, 2017 Accepted: October 29, 2017 Published: December 04, 2017
The purpose of this study was to investigate the mechanism of monocrotaline (MCT)-induced pulmonary artery hypertension (PAH) and determine whether 4-chloro-DL-phenylalanine (PCPA) could inhibit pulmonary arterial remodeling associated with connective tissue growth factor (CTGF) expression and downstream signal pathway. MCT was administered to forty Sprague Dawley rats to establish the PAH model. PCPA was administered at doses of 50 and 100 mg/kg once daily for 3 weeks via intraperitoneal injection. On day 22, the pulmonary arterial pressure (PAP), right ventricle hypertrophy index (RVI) and pulmonary artery morphology were assessed and the serotonin receptor-1B (SR-1B), CTGF, p-ERK/ERK were measured by western blot or immunohistochemistry. The concentration of serotonin in plasma was checked by ELISA. Apoptosis and apoptosis-related indexes were detected by TUNEL and western blot. In the MCT-induced PAH models, the PAP, RVI, pulmonary vascular remodeling, SR-1B index, CTGF index, anti-apoptotic factors bcl-xl and bcl-2, serotonin concentration in plasma were all increased and the pro-apoptotic factor caspase-3 was reduced. PCPA significantly ameliorated pulmonary arterial remodeling induced by MCT, and this action was associated with accelerated apoptosis and down-regulation of CTGF, SR-1B and p-ERK/ERK. The present study suggests that PCPA protects against the pathogenesis of PAH by suppressing remodeling and inducing apoptosis, which are likely associated with CTGF and downstream ERK signaling pathway in rats.
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