Clinical Research Papers:

Monitoring neoadjuvant therapy responses in rectal cancer using multimodal nonlinear optical microscopy

Lian-Huang Li, Zhi-Fen Chen, Xing-Fu Wang, Xing Liu, Wei-Zhong Jiang, Shuang-Mu Zhuo, Li-Wei Jiang, Guo-Xian Guan and Jian-Xin Chen _

PDF  |  HTML  |  How to cite

Oncotarget. 2017; 8:107323-107333. https://doi.org/10.18632/oncotarget.22366

Metrics: PDF 1446 views  |   HTML 1835 views  |   ?  


Lian-Huang Li1,*, Zhi-Fen Chen2,*, Xing-Fu Wang3,*, Xing Liu2, Wei-Zhong Jiang2, Shuang-Mu Zhuo1, Li-Wei Jiang1, Guo-Xian Guan2 and Jian-Xin Chen1

1Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, Fujian, China

2Department of Colorectal Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian, China

3Department of Pathology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China

*These authors contributed equally to this work

Correspondence to:

Jian-Xin Chen, email: [email protected]

Guo-Xian Guan, email: [email protected]

Keywords: neoadjuvant therapy, rectal cancer, tumor response, stromal response, nonlinear microscopy

Received: March 27, 2017     Accepted: August 27, 2017     Published: November 03, 2017


Most patients with rectal cancer have a better prognosis after receiving neoadjuvant therapy because of its remarkable curative effect. However, no device delivers real-time histopathologic information on treatment response to help clinicians tailor individual therapeutic strategies. We assessed the potential of multimodal nonlinear optical microscopy to monitor therapeutic responses, including tumoral and stromal responses. We found that two-photon excited fluorescence imaging can, without labeling, identify colloid response, inflammatory cell infiltration, vascular proliferation, and tumor regression. It can also directly detect rare residual tumor cells, which may be helpful for distinguishing tumor shrinkage from tumor fragmentation. In addition, second harmonic generation imaging shows the ability to monitor three types of fibrotic responses: mature, intermediate, and immature. We also determined nonlinear spectra, collagen density, and collagen orientation indexes to quantitatively analyze the histopathologic changes induced by neoadjuvant therapy in rectal cancer. Our work demonstrates that nonlinear optical microscopy has the potential to become a label-free, real-time, in vivo medical imaging technique and provides the groundwork for further exploration into the application of nonlinear optical microscopy in a clinical setting.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 22366