Oncotarget

Research Papers:

Molecular analyses of prostate tumors for diagnosis of malignancy on fine-needle aspiration biopsies

Menglin Shan, Qianlin Xia, Dong Yan, Yanjun Zhu, Xuan Zhang, Guihong Zhang, Jianming Guo, Jun Hou, Weiping Chen, Tongyu Zhu, Xiaoyan Zhang, Jianqing Xu, Jin Wang _, Tao Ding and Jianghua Zheng

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Oncotarget. 2017; 8:104761-104771. https://doi.org/10.18632/oncotarget.22289

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Abstract

Menglin Shan1,*, Qianlin Xia1,*, Dong Yan2,*, Yanjun Zhu3,*, Xuan Zhang1, Guihong Zhang1, Jianming Guo3, Jun Hou4, Weiping Chen5, Tongyu Zhu1, Xiaoyan Zhang1, Jianqing Xu1, Jin Wang1, Tao Ding6 and Jianghua Zheng1,7

1Shanghai Public Health Clinical Center, Fudan University, Jinshan, Shanghai, P.R. China

2Department of Medical Oncology, Beijing Chaoyang Hospital Affiliated to Capital Medical University, Beijing, P.R. China

3Department of Urology, Zhongshan Hospital, Fudan University, Yangpu, Shanghai, P.R. China

4Pathology, Zhongshan Hospital, Fudan University, Yangpu, Shanghai, P.R. China

5Genomics Core, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA

6Department of Urology, The Sixth People’s Hospital South Campus, Shanghai Jiao Tong University, Fengxian, Shanghai, P.R. China

7Department of Laboratory Medicine, Zhoupu Hospital Affiliated to Shanghai University of Medicine and Health Sciences, Pudong New Area, Shanghai, P.R. China

*These authors contributed equally to this work

Correspondence to:

Jin Wang, email: wangjin@shaphc.org

Tao Ding, email: dingtao1006@126.com

Jianghua Zheng, email: zhengjianghua2014@163.com

Keywords: prostate cancer; fine-needle aspiration; gene expression profiling; pathway analysis

Received: June 12, 2017     Accepted: October 15, 2017     Published: November 06, 2017

ABSTRACT

Prostate cancer (PCa) is a common cancer and remains the second-leading cause of cancer-associated mortality in men, but diagnosis of PCa remains a main clinical challenge. To investigate the involvement of differentially expressing genes in PCa with deregulated pathways to allow earlier diagnosis of the disease, transcriptomic analyses of differential expression genes in fine-needle aspiration (FNA) biopsies helped to discriminate PCa from benign prostatic hyperplasia (BPH). We identified 255 genes that were deregulated in prostate tumors compared with BPH tissues. qRT-PCR was conducted to examine the expression levels of the four genes in FNA biopsies and confirmed that ITGBL1 was significantly up-regulated and HOXA7, KRT15 and TGM4 were down-regulated in the PCa compared to the BPH, with a sensitivity of 87.1% and a specificity of 87.8%; the area under the receiver operating characteristic curve was estimated at 0.94, which was significantly improved compared with PSA alone (AUC = 0.82). Moreover, the increased expression of ITGBL1 correlated with total cholesterol, triglyceride and PSA. Our results demonstrated that transcriptomic analyses in FNA biopsies could facilitate rapid identification of potential targets for therapy and diagnosis of PCa.


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