Oncotarget

Research Papers:

This article has been corrected. Correction in: Oncotarget. 2020; 11:891-892.

Gene function analysis and underlying mechanism of esophagus cancer based on microarray gene expression profiling

Ying Yue, Mengjia Song, Yamin Qiao, Pupu Li, Yiqiang Yuan, Jingyao Lian, Suying Wang and Yi Zhang _

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Oncotarget. 2017; 8:105222-105237. https://doi.org/10.18632/oncotarget.22160

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Abstract

Ying Yue1,2,3,*, Mengjia Song1,2,*, Yamin Qiao1,2,*, Pupu Li1,2, Yiqiang Yuan3, Jingyao Lian1,2, Suying Wang4 and Yi Zhang1,2,5,6

1Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China

2Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China

3The No.7. People’s Hospital of Zhengzhou, Zhengzhou, Henan 450016, China

4Clinical Laboratory, Hebi People’s Hospital, Hebi 458030, China

5School of Life Sciences, Zhengzhou University, Zhengzhou, Henan 450001, China

6Key Laboratory for Tumor Immunology and Biotherapy of Henan Province, Zhengzhou, Henan 450052, China

*These authors have contributed equally to this work

Correspondence to:

Yi Zhang, email: yizhang@zzu.edu.cn

Keywords: esophagus cancer; gene function analysis; microarray gene expression profiling

Received: February 23, 2017    Accepted: August 28, 2017    Published: October 30, 2017

ABSTRACT

Esophageal cancer (EC) is one of the most common digestive malignant tumors worldwide. Over the past decades, there have been minimal improvements in outcomes for patients with EC. New targets and novel therapies are needed to improve outcomes for these patients. This study aimed to explore the molecular mechanisms of EC by integrated bioinformatic analyses of the feature genes associated with EC and correlative gene functions which can distinguish cancerous tissues from non-cancerous tissues. Gene expression profile GSE20347 was downloaded from Gene Expression Omnibus (GEO) database, including 17 EC samples and their paired adjacent non-cancerous samples. The differentially expressed genes (DEGs) between EC and normal specimens were identified and then applied to analyze the GO enrichment on gene functions and KEGG pathways. Corresponding Pathway Relation Network (Pathway-net) and Gene Signal Network (signal-net) of DEGs were established based on the data collected from GCBI datasets. The results showed that DEGs mainly participated in the process of cell adhesion, cell proliferation, survival, invasion, metastasis and angiogenesis. Aberrant expression of PTK2, MAPK signaling pathway, PI3K-Akt signaling pathway, p53 signaling pathway and MET were closely associated with EC carcinogenesis. Importantly, Interleukin 8 (IL8) and C-X-C chemokine receptor type 7 (CXCR-7) were predicted to be significantly related to EC. These findings were further validated by analyzing both TCGA database and our clinical samples of EC. Our discovery provides a registry of genes and pathways that are disrupted in EC, which has the potential to be used in clinic for diagnosis and target therapy of EC in future.


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