Oncotarget

Research Papers:

Calcium homeostasis and endoplasmic reticulum stress are involved in Salvianolic acid B-offered protection against cardiac toxicity of arsenic trioxide

Jing-Yi Zhang, Bin Zhang, Min Wang, Wei Wang, Ping Liao, Gui-Bo Sun _ and Xiao-Bo Sun

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Oncotarget. 2017; 8:97384-97393. https://doi.org/10.18632/oncotarget.22127

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Abstract

Jing-Yi Zhang1,2,3,4,*, Bin Zhang1,2,3,4,*, Min Wang1,2,3,4, Wei Wang1,2,3,4, Ping Liao5, Gui-Bo Sun1,2,3,4 and Xiao-Bo Sun1,2,3,4

1Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China

2Beijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese Medicine (Natural Medicine) and Translational Medicine, Beijing, China

3Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing, China

4Zhongguancun Open Laboratory of The Research and Development of Natural Medicine and Health Products, Beijing, China

5College of Pharmacy, Guilin Medical University, Guilin, China

*These authors have contributed equally to this work

Correspondence to:

Gui-Bo Sun, email: sunguibo@126.com

Xiao-Bo Sun, email: sun_xiaobo163@163.com

Keywords: arsenic trioxide; cardiotoxicity; salvianolic acid B; calcium homeostasis; endoplasmic reticulum stress

Received: January 10, 2017    Accepted: August 26, 2017    Published: October 26, 2017

ABSTRACT

Arsenic trioxide (ATO) is a potent anticancer agent used to treat acute promyelocytic leukemia. However, its cardiotoxicity limits ATO’s widespread clinical use. Previous studies demonstrated that ATO may aggravate Ca2+ overload and promote endoplasmic reticulum stress (ERS). Salvianolic acid B (Sal B) is cardioprotective against ATO and enhances ATO’s anticancer activities. The present study assessed whether the Sal B protective effect was related to maintenance of Ca2+ homeostasis and inhibition of ER stress. Male BALB/c mice were injected with ATO or ATO+Sal B once a day via the tail vein for 2 weeks. We then detected the effects of Sal B in real time using adult rat ventricular cardiomyocytes in vitro using an IonOptix MyoCam system. Sal B treatment alleviated ATO-induced abnormal cardiac contractions and Ca2+ homeostasis imbalance. Sal B increased sarcoplasmic reticulum Ca2+-ATPase (SERCA) activity, regulated Ca2+ handling protein expression, and decreased expression of ERS proteins. Our results demonstrate that the cardioprotective effect of Sal B correlates with SERCA modulation, maintenance of Ca2+ homeostasis, and inhibition of ER stress. These findings suggest Sal B may ameliorate ATO cardiotoxicity during clinical application.


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