Up-regulation of RNF187 induces hepatocellular carcinoma cell epithelial to mesenchymal transitions
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Song-Lin Yu1,*, Jin-Cai Wu2,*, Peng-Fei Liu1, Kai Liu1, Chun Ye1, Kai-Lun Zhou2, Zhuo-Ri Li2 and Ya-Ping Xu3
1Department of General Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China
2Department of Hepatobiliary Surgery and Organ Transplantation, Hainan Provincial People’s Hospital, University of South China, Haikou 570311, China
3Department of Gastroenterology Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China
*These authors contributed equally to this work
Ya-Ping Xu, email: email@example.com
Zhuo-Ri Li, email: firstname.lastname@example.org
Keywords: hepatocellular carcinoma, RNF187, EMT, prognosis
Received: April 21, 2017 Accepted: September 23, 2017 Published: October 19, 2017
Ring finger protein 187 (RNF187) has been identified to be a co-activator linking c Jun to Ras signaling. However, the expression and function of RNF187 in hepatocellular carcinomas (HCC) remains unclear. Here, we tried to determine the expression and roles of RNF187 in hepatocellular carcinomas (HCC).The expression of RNF187 was determined in HCC tissues and cell lines, and we found that RNF187 expressed highly in HCC tissues compared with the corresponding adjacent liver tissues both in mRNA and protein level, which was consistent with the result of immunohistochemistry on HCC tissue microarrays. In HCC cell lines, the level of RNF187 was positively associated with the HCC cells metastatic potential. By the RNF187 interference and cDNA transfection, we showed that the high level of RNF187 induced the HCC cells invasion and metastasis both in vitro and in vivo, as well as the high ability of colony formation.Mechanistically, we detected the high level of RNF187 promoted cell scatter by inducing epithelial-mesenchymal transition (EMT). Clinically, the high level of RNA187 was significantly correlated with a malignant phenotype, including larger tumor size, multiple tumors, and microvascular invasion. Importantly, high level of RNF187 correlated with HCC patients' shorter OS and lower disease free survival rates than those with low level of RNF187. Our results revealed that elevated expression of RNF187 induced hepatocellular carcinoma cell epithelial to mesenchymal transitions, and represented a novel marker for predicting the poor prognosis of HCC.
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