Bovine herpesvirus 1 tegument protein  UL21  plays critical roles in viral secondary envelopment and cell-to-cell spreading

Farzana Shahin; Sohail Raza; Kui Yang; Changmin Hu; Yingyu Chen; Huanchun Chen; Aizhen Guo

doi:10.18632/oncotarget.21776

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

Bovine herpesvirus 1 tegument protein UL21 plays critical roles in viral secondary envelopment and cell-to-cell spreading

Farzana Shahin, Sohail Raza, Kui Yang, Changmin Hu, Yingyu Chen, Huanchun Chen and Aizhen Guo _

PDF | HTML | Supplementary Files | How to cite

Oncotarget. 2017; 8:94462-94480. https://doi.org/10.18632/oncotarget.21776

Metrics: PDF 2069 views | HTML 2784 views | ?

Abstract

Farzana Shahin1,2, Sohail Raza1,2,6, Kui Yang3, Changmin Hu2, Yingyu Chen2, Huanchun Chen1,2 and Aizhen Guo1,2,4,5

1The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China

2College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China

3Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803, USA

4Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan 430070, China

5Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan 430070, China

6Department of Microbiology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan

Correspondence to:

Aizhen Guo, email: [email protected]

Keywords: BoHV-1, interacting proteins, secondary envelopment, tegument, UL21

Received: July 13, 2017 Accepted: September 21, 2017 Published: October 10, 2017

ABSTRACT

Bovine herpesvirus 1 (BoHV-1) UL21 is a tegument protein thought to be indispensable for efficient viral growth but its precise function in BoHV-1 is currently unknown. To determine the function of UL21 in BoHV-1 replication, we constructed a mutant virus bearing a UL21 deletion (vBoHV-1-ΔUL21) and its revertant virus, vBoHV-1-ΔUL21R, in which the UL21 gene was restored using a bacterial artificial chromosome system. The replication of vBoHV-1-ΔUL21 was 1,000-fold lower and its plaque size was 85% smaller than those of the wild-type virus (BoHV-1). An ultrastructural analysis showed that deletion of UL21 led to an un-enveloped capsid accumulation in the cytoplasm, whereas nucleocapsid egress was not impaired, suggesting that UL21 is critical for secondary envelopment in BoHV-1. Co-immunoprecipitation assays revealed that HA-tagged UL21 pulled down UL16, suggesting that these two proteins form a complex, and this was further confirmed by a co-immunofluorescence assay. Taken together, these data provide evidence that UL21 plays critical roles in BoHV-1 secondary envelopment, and UL16 is likely to be involved in these activities.

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 21776

Publication Alerts

Research Papers:

Bovine herpesvirus 1 tegument protein UL21 plays critical roles in viral secondary envelopment and cell-to-cell spreading

Abstract