Oncotarget

Research Papers:

Circular RNA hsa_circ_000984 promotes colon cancer growth and metastasis by sponging miR-106b

Xiao-Wu Xu, Bo-An Zheng, Zhi-Ming Hu, Zhen-Yuan Qian, Chao-Jie Huang, Xi-Qiang Liu and Wei-Ding Wu _

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Oncotarget. 2017; 8:91674-91683. https://doi.org/10.18632/oncotarget.21748

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Abstract

Xiao-Wu Xu1,*, Bo-An Zheng2,*, Zhi-Ming Hu3, Zhen-Yuan Qian1, Chao-Jie Huang1, Xi-Qiang Liu3 and Wei-Ding Wu3

1Department of Gastrointestinal and Pancreatic Surgery, Zhejiang Provincial People's Hospital, Key Laboratory of Gastroenterology of Zhejiang Provincial People's Hospital of Hangzhou Medical College, Hangzhou, 310014, Zhejiang, China

2Department of Colorectal Surgery, Zhejiang Provincial People's Hospital, Hangzhou, 310014, Zhejiang, China

3Department of Hepatobiliary-Pancreatic Surgery, Zhejiang Provincial People's Hospital, Hangzhou, 310014, Zhejiang, China

*These authors have contributed equally to this work

Correspondence to:

Wei-Ding Wu, email: Weidingwu@126.com

Xi-Qiang Liu, email: 2583749877@qq.com

Keywords: circular RNA, hsa_circ_000984, colon cancer, growth, miR-106b

Received: June 05, 2017     Accepted: July 30, 2017     Published: October 10, 2017

ABSTRACT

Circular RNAs (circRNAs) as a novel type of noncoding RNAs (ncRNAs) are widely studied in the development of human various diseases, including cancer. Here, we found circular RNA hsa_circ_000984 encoded by the CDK6 gene was remarkably upregulated in the tissues of colorectal cancer (CRC) patients and in the CRC cell lines. Moreover, high expression level of hsa_circ_000984 was significantly associated with advanced colorectal cancer. Further analysis revealed that hsa_circ_000984 knockdown could inhibit cell proliferation, migration, invasion in vitro and tumor formation in vivo in CRC cell lines. Mechanically, we found that hsa_circ_000984 may act as a competing endogenous RNA (ceRNA) by competitively binding miR-106b and effectively upregulate the expression of CDK6, thereby inducing a series of malignant phenotypes of tumor cells. Taken together, these observations suggest that the hsa_circ_000984 could mediate the expression of gene CDK6 by acting as a ceRNA, which may contribute to a better understanding of between the regulatory miRNA network and CRC pathogenesis.


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