Oncotarget

Research Papers:

NDRG4 promotes myogenesis via Akt/CREB activation

Mingfei Zhu, Rong Zheng, Yiwen Guo, Yunxia Zhang and Bo Zuo _

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Oncotarget. 2017; 8:101720-101734. https://doi.org/10.18632/oncotarget.21591

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Abstract

Mingfei Zhu1,*, Rong Zheng1,*, Yiwen Guo1, Yunxia Zhang3 and Bo Zuo1,2

1Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. China

2The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China

3College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou 466000, China

*These authors have contributed equally to this work

Correspondence to:

Bo Zuo, email: zuobo@mail.hzau.edu.cn

Keywords: myogenesis; NDRG4; CTMP; Akt; CREB

Received: July 28, 2017     Accepted: September 03, 2017     Published: October 06, 2017

ABSTRACT

N-Myc downstream-regulated gene 4 (NDRG4) plays an important role in biological processes and pathogenesis, but its function in muscle development is unclear. In this study, we investigated the function of the NDRG4 gene in the regulation of myogenic differentiation. NDRG4 expression is upregulated during muscle regeneration and C2C12 myoblast differentiation. Gain and loss of function studies revealed that NDRG4 dramatically promotes expression of myogenic differentiation factor (MyoD), myogenin (MyoG), and myosin heavy chain (MyHC) genes and myotube formation. Mechanistically, the binding of NDRG4 to carboxyl-terminal modulator protein (CTMP) abates the interaction of CTMP and protein kinase B (Akt) and increases the phosphorylation of Akt and cAMP response element binding protein (CREB), which leads to increased expression of myogenic genes. Our results reveal that NDRG4 promotes myogenic differentiation via Akt/CREB activation.


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