Research Papers:

Naringin improves random skin flap survival in rats

Liang Cheng, Tingxiang Chen, Qiming Tu, Hang Li, Zhenghua Feng, Zhijie Li and Dingsheng Lin _

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Oncotarget. 2017; 8:94142-94150. https://doi.org/10.18632/oncotarget.21589

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Liang Cheng1, Tingxiang Chen1, Qiming Tu1, Hang Li1, Zhenghua Feng1, Zhijie Li1 and Dingsheng Lin1

1Department of Hand and Plastic Surgery, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China

Correspondence to:

Dingsheng Lin, email: [email protected]

Zhijie Li, email: [email protected]

Keywords: naringin, random flap survival, angiogenesis, inflammation, rat

Received: July 28, 2017     Accepted: September 18, 2017     Published: October 06, 2017


Background: Random-pattern flap transfer is commonly used to treat soft-tissue defects. However, flap necrosis remains a serious problem. Naringin accelerates angiogenesis by activating the expression of vascular endothelial growth factor (VEGF). In the present study, we investigated whether naringin improves the survival of random skin flaps.

Results: Compared with controls, the naringin-treated groups exhibited significantly larger mean areas of flap survival, significantly increased SOD activity and VEGF expression, and significantly reduced MDA level. Hematoxylin and eosin (HE) staining revealed that naringin promoted angiogenesis and inhibited inflammation.

Materials and Methods: “McFarlane flap” models were established in 90 male Sprague-Dawley (SD) rats divided into three groups: a 40 mg/kg control group (0.5 % sodium carboxymethylcellulose), a 40 mg/kg naringin-treated group, and an 80 mg/kg naringin-treated group. The extent of necrosis was measured 7 days later, and tissue samples were subjected to histological analysis. Angiogenesis was evaluated via lead oxide–gelatin angiography, immunohistochemistry, and laser Doppler imaging. Inflammation was evaluated by measurement of serum TNF-α (tumor necrosis factor-α) and IL-6 (interleukin-6) levels. Oxidative stress was assessed by measuring superoxide dismutase (SOD) activity and the malondialdehyde (MDA) level.

Conclusion: Naringin improved random skin flap survival.

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