Oncotarget

Research Papers:

HAT1 induces lung cancer cell apoptosis via up regulating Fas

Na Han, Lei Shi, Qiuyun Guo, Wei Sun, Yang Yu, Li Yang, Xiaoxi Zhang and Mengxian Zhang _

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Oncotarget. 2017; 8:89970-89977. https://doi.org/10.18632/oncotarget.21205

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Abstract

Na Han1, Lei Shi1, Qiuyun Guo1, Wei Sun1, Yang Yu1, Li Yang1, Xiaoxi Zhang1 and Mengxian Zhang1

1Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

Correspondence to:

Mengxian Zhang, email: jz33445@163.com

Keywords: lung cancer, epithelial cells, HAT1, Fas, apoptosis

Received: July 03, 2017    Accepted: August 17, 2017    Published: September 23, 2017

ABSTRACT

The dysfunction of apoptosis is one of the factors contributing to lung cancer (LC) growth. Histone acetyltransferase HAT1 can up regulate cell apoptosis. This study aims to investigate the mechanism by which HAT1 induces LC cell (LCC) apoptosis via up regulating the expression of Fas. In this study, the surgically removed human LC tissues were collected. LCCs were isolated from the LC tissues and analyzed for the expression of HAT1 and Fas by RT-qPCR and Western blotting. We observed that the expression of Fas was negatively correlated with PAR2 in LCCs. Activation of PAR2 suppressed the expression of Fas in normal lung epithelial cells. The expression of HAT1 was lower and positively correlated with Fas expression and negatively correlated with PAR2 expression in LCCs. Activation of PAR2 suppressed Fas expression in lung epithelial cells via inhibiting HAT1. Restoration of HAT1 expression restored Fas expression in LCCs and induced LCC apoptosis. In conclusion, less expression of HAT1 in LCCs was associated with the pathogenesis of LC. Up regulation of HAT1 expression in LCCs can induce LCCs apoptosis, which may be a potential novel therapy for the treatment of LC.


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