Rapid, ultra low coverage copy number profiling of cell-free DNA as a precision oncology screening strategy
Metrics: PDF 2054 views | HTML 3989 views | ?
Daniel H. Hovelson1,2, Chia-Jen Liu1,3, Yugang Wang4, Qing Kang5, James Henderson4, Amy Gursky4, Scott Brockman1, Nithya Ramnath5, John C. Krauss5, Moshe Talpaz5, Malathi Kandarpa5, Rashmi Chugh5, Missy Tuck5, Kirk Herman5, Catherine S. Grasso10,11, Michael J. Quist10,11, Felix Y. Feng12, Christine Haakenson13, John Langmore13, Emmanuel Kamberov13, Tim Tesmer13, Hatim Husain14, Robert J. Lonigro1,3, Dan Robinson1,3,8, David C. Smith5,8, Ajjai S. Alva5,8, Maha H. Hussain5,8,15, Arul M. Chinnaiyan1,3,8,10, Muneesh Tewari2,5,6,7,8,9, Ryan E. Mills2,7, Todd M. Morgan1,4,8,* and Scott A. Tomlins1,3,4,8,*
1Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI, USA
2Department of Computational Medicine and Bioinformatics, University of Michigan Medical School, Ann Arbor, MI, USA
3Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA
4Department of Urology, University of Michigan Medical School, Ann Arbor, MI, USA
5Department of Internal Medicine (Hematology/Oncology), University of Michigan Medical School, Ann Arbor, MI, USA
6Department of Biomedical Engineering, University of Michigan Medical School, Ann Arbor, MI, USA
7Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI, USA
8Department of Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI, USA
9Department of Biointerfaces Institute, University of Michigan Medical School, Ann Arbor, MI, USA
10Division of Hematology-Oncology, University of California, Los Angeles and the Jonsson Comprehensive Cancer Center, Los Angeles, CA, USA
11The Parker Institute of Cancer Immunotherapy, San Francisco, CA, USA
12Departments of Radiation Oncology, Urology, and Medicine, University of California at San Francisco, San Francisco, CA, USA
13Takara Bio USA, Ann Arbor, MI, USA
14Medical Oncology, University of California, San Diego Moore’s Cancer Center, San Diego, CA, USA
15Present address: Division of Hematology/Oncology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA
Scott A. Tomlins, email: firstname.lastname@example.org
Todd M. Morgan, email: email@example.com
Keywords: cell-free DNA, precision oncology, prostate cancer, whole genome sequencing, copy-number analysis
Received: August 29, 2017 Accepted: September 08, 2017 Published: September 22, 2017
Current cell-free DNA (cfDNA) next generation sequencing (NGS) precision oncology workflows are typically limited to targeted and/or disease-specific applications. In advanced cancer, disease burden and cfDNA tumor content are often elevated, yielding unique precision oncology opportunities. We sought to demonstrate the utility of a pan-cancer, rapid, inexpensive, whole genome NGS of cfDNA approach (PRINCe) as a precision oncology screening strategy via ultra-low coverage (~0.01x) tumor content determination through genome-wide copy number alteration (CNA) profiling. We applied PRINCe to a retrospective cohort of 124 cfDNA samples from 100 patients with advanced cancers, including 76 men with metastatic castration-resistant prostate cancer (mCRPC), enabling cfDNA tumor content approximation and actionable focal CNA detection, while facilitating concordance analyses between cfDNA and tissue-based NGS profiles and assessment of cfDNA alteration associations with mCRPC treatment outcomes. Therapeutically relevant focal CNAs were present in 42 (34%) cfDNA samples, including 36 of 93 (39%) mCRPC patient samples harboring AR amplification. PRINCe identified pre-treatment cfDNA CNA profiles facilitating disease monitoring. Combining PRINCe with routine targeted NGS of cfDNA enabled mutation and CNA assessment with coverages tuned to cfDNA tumor content. In mCRPC, genome-wide PRINCe cfDNA and matched tissue CNA profiles showed high concordance (median Pearson correlation = 0.87), and PRINCe detectable AR amplifications predicted reduced time on therapy, independent of therapy type (Kaplan-Meier log-rank test, chi-square = 24.9, p < 0.0001). Our screening approach enables robust, broadly applicable cfDNA-based precision oncology for patients with advanced cancer through scalable identification of therapeutically relevant CNAs and pre-/post-treatment genomic profiles, enabling cfDNA- or tissue-based precision oncology workflow optimization.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.