Oncotarget

Research Papers:

Differential regulation of mRNAs and lncRNAs related to lipid metabolism in two pig breeds

Wanlong Huang, Xiuxiu Zhang, Ai Li, Lingli Xie and Xiangyang Miao _

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Oncotarget. 2017; 8:87539-87553. https://doi.org/10.18632/oncotarget.20978

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Abstract

Wanlong Huang1, Xiuxiu Zhang1, Ai Li1, Lingli Xie1 and Xiangyang Miao1

1State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Correspondence to:

Xiangyang Miao, email: [email protected], [email protected]

Keywords: lncRNA, subcutaneous adipose tissue, fat deposition, pig, RNA-seq

Received: June 30, 2017    Accepted: August 27, 2017    Published: September 18, 2017

ABSTRACT

Long non-coding RNAs (lncRNAs) can regulate lipid metabolism and adipogenesis. However, there is little research on the role of lncRNAs in fat deposition in pig. In this study, RNA-seq technology was used to analyze the gene expression profiles of subcutaneous adipose tissue in Laiwu (LW) and Large White (LY) pigs. Then, key lncRNAs and genes associated with lipid metabolism and adipogenic differentiation were identified. Fifty four lncRNAs and 482 known mRNAs were differentially expressed in the two pig breeds. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses revealed that differentially expressed genes and the target genes of differentially expressed lncRNAs were significantly enriched in PPAR signaling pathway and biological processes including fat cell differentiation and fatty acid metabolism. Key lncRNAs might regulate adipogenic differentiation and fatty acid metabolism by regulating genes involved in above signaling pathway and biological processes. Specifically, XLOC_014379, XLOC_011279, XLOC_064871, XLOC_019518 and XLOC_013639 might target SCD, LPIN1, TRIB3, EGR2 and FABP3, respectively, and then play critical regulatory role. These results are useful for understanding fat deposition in pig, breeding livestock with high quality meat, and preventing and treating lipid metabolic disease.


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