Oncotarget

Research Papers:

Aspirin exerts high anti-cancer activity in PIK3CA-mutant colon cancer cells

Mancang Gu, Reiko Nishihara, Yang Chen, Wanwan Li, Yan Shi, Yohei Masugi, Tsuyoshi Hamada, Keisuke Kosumi, Li Liu, Annacarolina da Silva, Jonathan A. Nowak, Tyler Twombly, Chunxia Du, Hideo Koh, Wenbin Li, Jeffrey A. Meyerhardt, Brian M. Wolpin, Marios Giannakis, Andrew J. Aguirre, Adam J. Bass, David A. Drew, Andrew T. Chan, Charles S. Fuchs, Zhi Rong Qian _ and Shuji Ogino

PDF  |  HTML  |  Supplementary Files  |  How to cite

Oncotarget. 2017; 8:87379-87389. https://doi.org/10.18632/oncotarget.20972

Metrics: PDF 2505 views  |   HTML 3615 views  |   ?  


Abstract

Mancang Gu1,2, Reiko Nishihara1,3,4,5,6, Yang Chen1,7, Wanwan Li1, Yan Shi1,7, Yohei Masugi1, Tsuyoshi Hamada1, Keisuke Kosumi1, Li Liu1,3, Annacarolina da Silva1, Jonathan A. Nowak6, Tyler Twombly1, Chunxia Du1, Hideo Koh1, Wenbin Li1, Jeffrey A. Meyerhardt8, Brian M. Wolpin8, Marios Giannakis8, Andrew J. Aguirre8, Adam J. Bass8,9,10, David A. Drew11,12, Andrew T. Chan11,12, Charles S. Fuchs13,14,15,*, Zhi Rong Qian1,6,* and Shuji Ogino1,4,6,*

1Department of Oncologic Pathology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA, USA

2College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, P.R. China

3Department of Nutrition, Harvard T.H. Chan School of Public Health, Boston, MA, USA

4Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, USA

5Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA

6Program in MPE Molecular Pathological Epidemiology, Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, USA

7Medical Oncology Department 2, Chinese People’s Liberation Army General Hospital, Beijing, P.R. China

8Department of Medical Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA, USA

9Broad Institute of MIT and Harvard, Cambridge, MA, USA

10Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA

11Clinical and Translational Epidemiology Unit, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA

12Division of Gastroenterology, Massachusetts General Hospital, Boston, MA, USA

13Yale Cancer Center, New Haven, CT, USA

14Department of Medicine, Yale School of Medicine, New Haven, CT, USA

15Smilow Cancer Hospital, New Haven, CT, USA

*These authors contributed equally to this work

Correspondence to:

Zhi Rong Qian, email: [email protected]

Shuji Ogino, email: [email protected]

Keywords: anti-tumor effect, colorectal cancer, isogenic cell model, NSAID, PI3K

Received: May 31, 2017     Accepted: August 31, 2017     Published: September 18, 2017

ABSTRACT

Evidence suggests that nonsteroidal anti-inflammatory drug aspirin (acetylsalicylic acid) may improve patient survival in PIK3CA-mutant colorectal carcinoma, but not in PIK3CA-wild-type carcinoma. However, whether aspirin directly influences the viability of PIK3CA-mutant colon cancer cells is poorly understood. We conducted in vitro experiments to test our hypothesis that the anti-proliferative activity of aspirin might be stronger for PIK3CA-mutant colon cancer cells than for PIK3CA-wild-type colon cancer cells. We measured the anti-proliferative effect of aspirin at physiologic concentrations in seven PIK3CA-mutant and six PIK3CA-wild-type human colon cancer cell lines. After exposure to aspirin, the apoptotic index and cell cycle phase of colon cancer cells were assessed. In addition, the effect of aspirin was examined in parental SW48 cells and SW48 cell clones with individual knock-in PIK3CA mutations of either c.3140A>G (p.H1047R) or c.1633G>A (p.E545K). Aspirin induced greater dose-dependent loss of cell viability in PIK3CA-mutant cells than in PIK3CA-wild-type cells after treatment for 48 and 72 hours. Aspirin treatment also led to higher proportions of apoptotic cells and G0/G1 phase arrest in PIK3CA-mutant cells than in PIK3CA-wild-type cells. Aspirin treatment of isogenic SW48 cells carrying a PIK3CA mutation, either c.3140A>G (p.H1047R) or c.1633G>A (p. E545K), resulted in a more significant loss of cell viability compared to wild-type controls. Our findings indicate that aspirin causes cell cycle arrest, induces apoptosis, and leads to loss of cell viability more profoundly in PIK3CA-mutated colon cancer cells than in PIK3CA-wild-type colon cancer cells. These findings support the use of aspirin to treat patients with PIK3CA-mutant colon cancer.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 20972