Oncotarget

Research Papers:

LncRNA HSP90AA1-IT1 promotes gliomas by targeting miR-885-5p-CDK2 pathway

Taihong Gao, Guangyan Gu, Jingxia Tian, Rui Zhang, Xiangrong Zheng, Yanan Wang, Qi Pang _ and Qian Liu

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Oncotarget. 2017; 8:75284-75297. https://doi.org/10.18632/oncotarget.20777

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Abstract

Taihong Gao1, Guangyan Gu2, Jingxia Tian3, Rui Zhang1, Xiangrong Zheng1, Yanan Wang2, Qi Pang1 and Qian Liu2

1Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China

2Department of Histology and Embryology, Shandong University School of Medicine, Jinan 250012, Shandong, China

3Department of Gynecology and Obstetrics, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China

Correspondence to:

Qi Pang, email: pangqi@sdu.edu.cn

Qian Liu, email: cardioqian@126.com

Keywords: lncRNA, HSP90AA1-IT1, miR-885-5p, CDK2, glioma

Received: June 08, 2017    Accepted: July 30, 2017    Published: September 08, 2017

ABSTRACT

It is well established that ncRNAs are emerging as important regulators in various types of cancers, however, their functions and contributions in cancers remain insufficiently defined. In this study, we reported the expression levels of a long noncoding RNA (lncRNA), named HSP90AA1-IT1 (HSP90AA1 intronic transcript 1), appeared to correlate with the pathological grades of gliomas and high level of HSP90AA1-IT1 indicated poor prognosis. Downregulation of HSP90AA1-IT1 in the glioma cell lines significantly suppressed cell viability, proliferation, EMT, invasion and migration in addition to an increase in apoptosis and aberrant cell cycle progression. The tumorigenic capacity of these cells in vivo were also inhibited. We further demonstrated that the oncogenic effects of HSP90AA1-IT1 could be mediated by a direct binding to miR-885-5p. Sharing the same binding sites with CDK2, a key regulator in gliomagenesis, HSP90AA1-IT1 competitively bound to miR-885-5p, thereby prevented CDK2 from miR-885-5p mediated post-transcriptional repression. Taken together, it is concluded that HSP90AA1-IT1, performs its function via regulating the development of gliomas through miR-885-5p-CDK2 signaling axis, and this has added new perspective to its role in tumorigenesis, thus providing potential therapeutic targets for glioma treatment.


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