The significance of lumican expression in ovarian cancer drug-resistant cell lines
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Andrzej Klejewski1,2, Karolina Sterzyńska3, Karolina Wojtowicz3, Monika Świerczewska3, Małgorzata Partyka3, Maciej Brązert4, Michał Nowicki3, Maciej Zabel3,5 and Radosław Januchowski3
1Department of Nursing, Poznań University of Medical Sciences, Poznań, Poland
2Department of Obstetrics and Womens Diseases, Poznań University of Medical Sciences, Poznań, Poland
3Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland
4Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznań University of Medical Sciences, Poznań, Poland
5Department of Histology and Embryology, Wrocław Medical University, Wrocław, Poland
Andrzej Klejewski, email: firstname.lastname@example.org
Keywords: drug resistance, ovarian cancer, lumican, collagen
Received: April 28, 2017 Accepted: June 30, 2017 Published: August 10, 2017
Purpose: The aim of the present study is to determine the expression of LUM in drug-resistant ovarian cancer cell lines.
Methods: Doxorubicin- (DOX), topotecan- (TOP) and vincristine- (VIN) resistant variants of the W1 ovarian cancer cell line were used in this study. We used quantitative real-time polymerase chain reactions to determine LUM mRNA levels. Protein expression was detected using Western blot and immunocytochemistry assays. Protein glycosylation was investigated using PGNase F digestion. Immunohistochemistry assays were used to determine protein expression in ovarian cancer patients.
Results: We observed increased expression of LUM in drug-resistant cell lines at both the mRNA and the protein level. The most abundant LUM expression was observed in TOP-resistant cell line. We observed LUM bands that corresponded to different molecular masses, and the most abundant LUM form was the secreted form, which had a mass of 50 kDa. Double immunofluorescence analysis showed co-expression of LUM and COL3A1 as well as the presence of extracellular COL3A1 in the TOP-resistant cell line. Finally, we detected the LUM protein in ovarian cancer tissue.
Conclusion: The expression of LUM in cytostatic-resistant cell lines suggests its role in drug resistance. The co-expression of LUM and COL3A1 indicates the significance of LUM in collagen fibre assembly. Expression in ovarian cancer tissue suggests that LUM can play a role in ovarian cancer pathogenesis in ways similar to other cancers.
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