Anti-proliferative and pro-apoptotic effects induced by simultaneous inactivation of HER1 and HER2 through endogenous polyclonal antibodies
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Narjara González Suárez1,*, Gretchen Bergado Báez1,*, Mabel Cruz Rodríguez1, Amelia Gutiérrez Pérez1, Lisset Chao García1, Diana Rosa Hernández Fernández1, Judith Raymond Pous2 and Belinda Sánchez Ramírez1
1Tumor Immunology Direction, Molecular Immunology Institute, Center of Molecular Immunology, Havana 11600, Cuba
2System Biology Direction, Molecular Immunology Institute, Center of Molecular Immunology, Havana 11600, Cuba
*These authors have contributed equally to this work
Belinda Sánchez Ramírez, email: [email protected]
Keywords: HER1, HER2, polyclonal antibodies, receptor degradation, apoptosis
Received: August 26, 2016 Accepted: March 22, 2017 Published: August 03, 2017
The human epidermal growth factor receptor (HER1) and its partner HER2 are extensively described oncogenes and validated targets for cancer therapy. However, the effectiveness of monospecific therapies targeting these receptors is hampered by resistance emergence, which is frequently associated with the upregulation of other members of HER family. Combined therapies using monoclonal antibodies or tyrosine kinase inhibitors have been suggested as a promising strategy to circumvent this resistance mechanism. We propose an alternative approach based on simultaneous inactivation of HER1 and HER2 by multi-epitope blockade with specific polyclonal antibodies induced by vaccination. Elicited antibodies impaired both receptors activation and induced their degradation, which caused the inhibition of down-signaling cascades. This effect was translated into cell cycle arrest and apoptosis induction of human tumor cells. Elicited antibodies were able to reduce the viability of a panel of human tumor lines with differential expression levels of HER1 and HER2. The most significant effects were obtained in the tumor lines with lower expression levels of both receptors. These new insights would contribute to the rational design of HER receptors targeting multivalent vaccines, as an encouraging approach for the treatment of cancer patients.
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