Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells
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Marina O. Gomzikova1, Margarita N. Zhuravleva1, Regina R. Miftakhova1, Svetlana S. Arkhipova1, Vladimir G. Evtugin1, Svetlana F. Khaiboullina1,2, Andrey P. Kiyasov1, Jenny L. Persson3,4, Nigel P. Mongan5,6, Richard G. Pestell7,8 and Albert A. Rizvanov1
1Kazan Federal University, Kazan 420008, Russia
2Department of Microbiology and Immunology, University of Nevada, Reno, NV 89557, USA
3Department of Translational Medicine, Lund University, Malmö 205 02, Sweden
4Department of Molecular Biology, Umeö University, Umeö 901 87, Sweden
5Cancer Biology and Translational Research, School of Veterinary Medicine and Science, University of Nottingham, Nottingham LE12 5RD, UK
6Department of Pharmacology, Weill Cornell Medicine, New York, NY 10065, USA
7Pennsylvania Cancer and Regenerative Medicine Research Center, Baruch S. Blumberg Institute, Pennsylvania Biotechnology Center, Wynnewood, PA 19096, USA
8Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 637551, Singapore
Albert A. Rizvanov, email: Albert.Rizvanov@kpfu.ru
Keywords: extracellular vesicles, membrane vesicles, cytochalasin B-induced membrane vesicles, angiogenesis, cell-free therapy
Received: March 15, 2017 Accepted: June 17, 2017 Published: July 31, 2017
Naturally occurring extracellular vesicles (EVs) play essential roles in intracellular communication and delivery of bioactive molecules. Therefore it has been suggested that EVs could be used for delivery of therapeutics. However, to date the therapeutic application of EVs has been limited by number of factors, including limited yield and full understanding of their biological activities. To address these issues, we analyzed the morphology, molecular composition, fusion capacity and biological activity of Cytochalasin B-induced membrane vesicles (CIMVs). The size of these vesicles was comparable to that of naturally occurring EVs. In addition, we have shown that CIMVs from human SH-SY5Y cells contain elevated levels of VEGF as compared to the parental cells, and stimulate angiogenesis in vitro and in vivo.
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