Oncotarget

Research Papers:

MiR-324-5p assists ultrasonography in predicting lymph node metastasis of unifocal papillary thyroid microcarcinoma without extracapsular spread

Yanhua Yang, Shujun Xia, Xiaofeng Ni, Zhongxin Ni, Lu Zhang, Wenhan Wang, Yanjun Kong, Yan Wang, Lei Ye and Weiwei Zhan _

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Oncotarget. 2017; 8:83802-83816. https://doi.org/10.18632/oncotarget.19717

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Abstract

Yanhua Yang1,2,*, Shujun Xia2,*, Xiaofeng Ni2, Zhongxin Ni2, Lu Zhang2, Wenhan Wang2, Yanjun Kong2, Yan Wang2, Lei Ye3 and Weiwei Zhan2

1Department of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

2Department of Ultrasonography, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

3Department of Endocrinology and Metabolism, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

*These authors have contributed equally to this work

Correspondence to:

Weiwei Zhan, email: [email protected]

Keywords: papillary thyroid carcinoma, lymph node metastasis, miR-324-5p, ultrasonography, fine needle aspiration

Received: March 29, 2016    Accepted: June 30, 2017    Published: July 31, 2017

ABSTRACT

Ultrasonography is the first choice of lymph node metastasis (LNM) detection which is crucial for therapeutic options of papillary thyroid cancer (PTC). However, the sensitivity of ultrasonography in detecting LNM of PTC is relatively low; especially in central LNM. MiR-324-5p has been reported to play important roles in the metastasis of various cancers. To explore the relationship between miR-324-5p and LNM in PTC, quantitative real-time polymerase chain reaction was performed in PTC tissue and fine needle aspiration (FNA) washout successively. Its correlation with LNM of PTC was analyzed. The clinicopathological and sonographic factors relating to LNM were also studied. Additionally, the function assay of miR-324-5p in PTC cells was conducted. Current study demonstrated that age was an independent protective factor and multifocality, advanced TNM stage, increased transverse diameter of thyroid nodule, ultrasound suspected LNM were independent risk factors of LNM. MiR-324-5p promoted proliferation, migration and invasion of PTC cell line. MiR-324-5p could serve as a candidate predictor along with ultrasonography in predicting LNM, especially central LNM of unifocal papillary thyroid microcarcinoma without extracapsular spread.


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