Oncotarget

Research Papers:

Effective protection against acute respiratory distress syndrome/sepsis injury by combined adipose-derived mesenchymal stem cells and preactivated disaggregated platelets

Chih-Hung Chen, Yung-Lung Chen, Pei-Hsun Sung, Cheuk-Kwan Sun, Kuan-Hung Chen, Yi-Ling Chen, Tien-Hung Huang, Hung-I Lu, Fan-Yen Lee, Jiunn-Jye Sheu, Sheng-Ying Chung, Mel S. Lee and Hon-Kan Yip _

PDF  |  HTML  |  How to cite

Oncotarget. 2017; 8:82415-82429. https://doi.org/10.18632/oncotarget.19312

Metrics: PDF 2856 views  |   HTML 2053 views  |   ?  


Abstract

Chih-Hung Chen1, Yung-Lung Chen2, Pei-Hsun Sung2, Cheuk-Kwan Sun3, Kuan-Hung Chen4, Yi-Ling Chen2, Tien-Hung Huang2, Hung-I Lu5, Fan-Yen Lee5, Jiunn-Jye Sheu5, Sheng-Ying Chung2, Mel S. Lee6,* and Hon-Kan Yip2,7,8,9,10,*

1Divisions of General Medicine, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

2Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

3Department of Emergency Medicine, E-Da Hospital, I-Shou University School of Medicine for International Students, Kaohsiung, Taiwan

4Department of Anesthesiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

5Division of Thoracic and Cardiovascular Surgery, Department of Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

6Department of Orthopedics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

7Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan

8Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan

9Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan

10Department of Nursing, Asia University, Taichung, Taiwan

*Both authors contributed equally to this work

Correspondence to:

Hon-Kan Yip, email: [email protected]

Mel S. Lee, email: [email protected]

Keywords: acute respiratory distress syndrome, sepsis, inflammation, adipose-derived mesenchymal stem cell, platelets

Received: October 11, 2016     Accepted: March 06, 2017     Published: July 17, 2017

ABSTRACT

This study assessed whether combining adipose-derived mesenchymal stem cells (ADMSC) with preactivated, disaggregated shape-changed platelets (PreD-SCP) was superior to either therapy alone for protecting rat lung from acute respiratory distress syndrome (ARDS) complicated by sepsis. ARDS and sepsis were induced through 100% oxygen inhalation and peritoneal administration of 1.5 mg/kg lipopolysaccharide (LPS), respectively. Adult-male Sprague-Dawley rats (n=40) were randomized into sham-control (SC), ARDS-LPS, ARDS-LPS-ADMSC (1.2x106 cells), ARDS-LPS-PreD-SCP (3.0x108, intravenous administration), and ARDS-LPS-ADMS/PreD-SCP groups, and were sacrificed 72 h after 48 h ARDS induction. Lung injury scores (LIS) and collagen deposition were highest in ARDS-LPS, lowest in SC, higher in ARDS-LPS+ADMSC than in ARDS-LPS+PreD-SCP and ARDS-LPS+ADMS/PreD-SCP, and higher in ARDS-LPS+PreD-SCP than in ARDS-LPS+ADMS/PreD-SCP (all p<0.0001). Alveolar-sac numbers, oxygen saturation, endothelial marker levels, and mitochondrial cytochrome-C levels exhibited opposite patterns with respect to LIS (all p<0.001). Levels of inflammatory, oxidative-stress, apoptosis, mitochondrial/DNA damage, and MAPK and Akt signaling markers exhibited patterns identical to that of LIS (all p<0.001). Anti-oxidant and anti-inflammatory protein levels increased progressively from SC to ARDS-LPS+ADMS/PreD-SCP (all p<0.0001). These findings indicate combined ADMSC/PreD-SCP was superior to either therapy alone for protecting rat lung from ARDS-sepsis injury.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 19312