MicroRNA-18a promotes proliferation and metastasis in hepatocellular carcinoma via targeting KLF4
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Li Liu1,*, Xun Cai2,*, Enqiang Liu3, Xia Tian4 and Chuan Tian4
1Department of Medicine & Appliance, Yunyan District Market Supervision and Administration Bureau, Guizhou 550001, China
2Department of Oncology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, China
3Department of Oncology, Qianjiang Central Hospital of Chongqing Municipality, Chongqing 409000, China
4Department of Nuclear Medicine, Guizhou Provincial People’s Hospital, Guizhou 550000, China
*These authors have contributed equally to this work
Chuan Tian, email: [email protected]
Keywords: miR-18a, KLF4, p21, hepatocellular carcinoma
Received: March 22, 2017 Accepted: June 19, 2017 Published: July 17, 2017
MicroRNAs (miRNAs) are short, non-coding and endogenous RNAs that played as important roles in the proliferation and metastasis of tumors. In this study, we determined the role of miR-18a in the regulation of HCC cell motility. We showed that miR-18a expression was upregulated in human HCC tissues and cell lines. Moreover, Elevated expression of miR-18a promoted the HCC cell proliferation and migration. KLF4 was identified as a direct target of miR-18a in HCC cells. Furthermore, overexpression of KLF4 attenuated the effects of miR-18a on the regulation of HCC cell motility. The expression of KLF4 was negatively associated with the expression of miR-18a expression in HCC tissues. We also showed that the cell cycle inhibitor p21 was aberrantly downregulated in HCC cells, whereas this inhibition was reversed by miR-18a inhibitor. These data indicated that miR-18a may play a positive role in hepatocellular carcinoma by promoting the proliferation and migration of HCC cells through targeting KLF4 as well as downstream p21.
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