Clinical Research Papers:

Nanobubbles as ultrasound contrast agent for facilitating small cell lung cancer imaging

Jin-Ping Wang, Xiao-Lin Zhou, Ji-Ping Yan, Rong-Qin Zheng and Wei Wang _

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Oncotarget. 2017; 8:78153-78162. https://doi.org/10.18632/oncotarget.18155

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Jin-Ping Wang1,2, Xiao-Lin Zhou3, Ji-Ping Yan2, Rong-Qin Zheng4 and Wei Wang1

1 Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, Shanxi, China

2 Department of Ultrasound, Shanxi Provincial People’s Hospital, Taiyuan, Shanxi, China

3 Radiation Medicine Laboratory, Institute of Radiation and Environmental Medicine, China Institute for Radiation Protection, Taiyuan, Shanxi, China

4 Department of Ultrasound, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, China

Correspondence to:

Wei Wang, email:

Rong-Qin Zheng, email:

Keywords: small cell lung cancer, nanobubbles, contrast-enhanced ultrasound, peak time, area under the curve and peak intensity

Received: March 07, 2017 Accepted: May 09, 2017 Published: May 24, 2017


Background: This study is to investigate whether liposome-loaded nanobubbles (NBs) have the potentials to carry anti-pro-gastrin releasing peptide (proGRP) antibody and enhance ultrasound imaging of small cell lung cancer (SCLC).

Methods: NBs were loaded with an antibody against SCLC (H446 cell line). A nude mouse model of SCLC tumor was established by a subcutaneous injection of tumor cell suspension in the dorsal skin. Images for contrast-enhanced ultrasound (CEUS) of xenograft tumors in the model were obtained through an intravenous injection of blank and targeting NBs.

Results: The targeted NBs showed a high binding affinity (90.2 ± 3.24%) of the H446 cells in vitro as compared to the blank NBs that have no affinity of the cells. In process of tumor imaging, no mice died of the NB application. CEUS imaging of the targeted NBs manifested significant increases in half-peak time, area under the curve and peak intensity as compared to the blank NBs. In the model of SCLC, treatment with targeting NBs resulted in a large amount of fluorescent dye accumulated in the tumor tissue but not the liver tissue.

Conclusion: Our results indicate that NBs can carry antibody traveling to the SCLC cells, whereas application of NBs is safe and reliable in serving as ultrasound contrast agents for improving SCLC imaging.

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