Oncotarget

Priority Research Papers:

An AML1-ETO/miR-29b-1 regulatory circuit modulates phenotypic properties of acute myeloid leukemia cells

Sayyed K. Zaidi _, Andrew W. Perez, Elizabeth S. White, Jane B. Lian, Janet L. Stein and Gary S. Stein

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Oncotarget. 2017; 8:39994-40005. https://doi.org/10.18632/oncotarget.18127

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Abstract

Sayyed K. Zaidi1, Andrew W. Perez1, Elizabeth S. White1, Jane B. Lian1, Janet L. Stein1 and Gary S. Stein

1 Department of Biochemistry and University of Vermont Cancer Center, The University of Vermont Larner College of Medicine, Burlington, VT, USA

Correspondence to:

Sayyed K. Zaidi, email:

Keywords: RUNX, leukemia, miR-29, AML1-ETO, t(8;21)

Received: April 18, 2017 Accepted: April 24, 2017 Published: May 24, 2017

Abstract

Acute myeloid leukemia (AML) is characterized by an aggressive clinical course and frequent cytogenetic abnormalities that include specific chromosomal translocations. The 8;21 chromosomal rearrangement disrupts the key hematopoietic RUNX1 transcription factor, and contributes to leukemia through recruitment of co-repressor complexes to RUNX1 target genes, altered subnuclear localization, and deregulation of the myeloid gene regulatory program. However, a role of non-coding microRNAs (miRs) in t(8;21)-mediated leukemogenesis is minimally understood. We present evidence of an interplay between the tumor suppressor miR-29b-1 and the AML1-ETO (also designated RUNX1-RUNX1T1) oncogene that is encoded by the t(8;21). We find that AML1-ETO and corepressor NCoR co-occupy the miR-29a/b-1 locus and downregulate its expression in leukemia cells. Conversely, re-introduction of miR-29b-1 in leukemia cells expressing AML1-ETO causes significant downregulation at the protein level through direct targeting of the 3’ untranslated region of the chimeric transcript. Restoration of miR-29b-1 expression in leukemia cells results in decreased cell growth and increased apoptosis. The AML1-ETO-dependent differentiation block and transcriptional program are partially reversed by miR-29b-1. Our findings establish a novel regulatory circuit between the tumor-suppressive miR-29b-1 and the oncogenic AML1-ETO that controls the leukemic phenotype in t(8;21)-carrying acute myeloid leukemia.


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