Oncotarget

Research Papers:

Prognostic values of tissue factor and its alternatively splice transcripts in human gastric cancer tissues

Min Wu, Lujun Chen, Ting Xu, Bin Xu, Jingting Jiang _ and Changping Wu

PDF  |  HTML  |  How to cite  |  Order a Reprint

Oncotarget. 2017; 8:53137-53145. https://doi.org/10.18632/oncotarget.17942

Metrics: PDF 1026 views  |   HTML 1263 views  |   ?  


Abstract

Min Wu1,2,3,*, Lujun Chen1,2,4,*, Ting Xu1,2,3,*, Bin Xu1,2,4, Jingting Jiang1,2,4 and Changping Wu1,2,4,5

1Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University, Jiangsu Changzhou 213003, China

2Research Center for Cancer Immunotherapy of Jiangsu Province, The Third Affiliated Hospital of Soochow University, Jiangsu Changzhou 213003, China

3Department of Rheumatology, The Third Affiliated Hospital of Soochow University, Jiangsu Changzhou 213003, China

4Institute of Cell Therapy, The Third Affiliated Hospital of Soochow University, Jiangsu Changzhou 213003, China

5Department of Oncology, The Third Affiliated Hospital of Soochow University, Jiangsu Changzhou 213003, China

*These authors have contributed equally to this work

Correspondence to:

Jingting Jiang, email: jiangjingting@suda.edu.cn

Changping Wu, email: wcpjjt@163.com

Keywords: gastric cancer, tissue factor, alternative splice, prognosis

Received: September 26, 2016    Accepted: February 21, 2017    Published: May 16, 2017

ABSTRACT

We have previously reported that the higher expression of TF in human esophageal cancer tissues was significantly associated with tumor invasion, intratumoral microvessel density and patients’ postoperative prognoses. Besides its trans-membranous form, TF also has alternatively spliced transcripts. In the present study, the transcripts of the two TF isoforms, flTF and asTF, in human gastric cancer tissues were determined by real-time PCR, and the correlation between the expression of TF isoforms and patient’s clinicopathological features was also analyzed. Our results showed that the relative mRNA expression levels of flTF and asTF in human gastric cancer tissues was significantly higher than those in normal tissues (P=0.035 and P=0.006, respectively). The relative mRNA expression level of asTF was significantly associated with age (P=0.018), meanwhile, we could not find that flTF or asTF expression level was correlated with any other characteristics of the patients, including gender, TNM stage, pathological grade, tumor size, histological type, or chemotherapy sensitivity. Univariate analysis demonstrated that the overall survival rate of gastric cancer patients with lower flTF or asTF expression level was greater than those with higher expression level (P=0.018 and =0.038, respectively). Multivariate COX model analysis also demonstrated that flTF expression (P=0.048) or asTF expression (P=0.002) could be used as independent prognostic predictors in human gastric cancer. Thus, both flTF and asTF mRNA expression levels in cancer tissues could be used as useful risk factors for evaluating the prognoses of patients suffering from gastric cancer.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 17942