Crosstalk between tongue carcinoma cells, extracellular vesicles, and immune cells in in vitro and in vivo models
Metrics: PDF 1008 views | HTML 1460 views | ?
Ahmed Al-Samadi1, Shady Adnan Awad2,3,*, Katja Tuomainen1,4,*, Yue Zhao1, Abdelhakim Salem5, Mataleena Parikka6,7 and Tuula Salo1,8,9
1Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki, Helsinki, Finland
2Hematology Research Unit, Department of Hematology, University of Helsinki and Helsinki University Central Hospital Comprehensive Cancer Center, Helsinki, Finland
3Clinical Pathology Department, National Cancer Institute, Cairo University, Cairo, Egypt
4Department of Otorhinolaryngology, Helsinki University Hospital, Helsinki, Finland
5Department of Internal Medicine, Clinicum, University of Helsinki, Helsinki, Finland
6Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland
7Oral and Maxillofacial Unit, Tampere University Hospital, Tampere, Finland
8Cancer and Translational Medicine Research Unit, University of Oulu, Oulu, Finland
9Medical Research Center, Oulu University Hospital, Oulu, Finland
*These authors have contributed equally in this work
Ahmed Al-Samadi, email: firstname.lastname@example.org
Keywords: tongue cancer, immune cells, extracellular vesicles, in vitro model, cytotoxicity
Received: December 14, 2016 Accepted: April 19, 2017 Published: May 10, 2017
The crosstalk between immune cells, cancer cells, and extracellular vesicles (EVs) secreted by cancer cells remains poorly understood. We created three-dimensional (3D) cell culture models using human leiomyoma discs and Myogel to study the effects of immune cells on highly (HSC-3) and less (SCC-25) invasive oral tongue squamous cell carcinoma (OTSCC) cell lines. Additionally, we studied the effects of EVs isolated from these cell lines on the cytotoxicity of CD8+ T and NK cells isolated from three healthy donors. Our analysis included the effects of these EVs on innate immunity in zebrafish larvae. Activated immune cells significantly decreased the proliferation of both OTSCC cell lines and associated with a diminished invasion area of HSC-3 cells. In general, EVs from SCC-25 increased the cytotoxic activity of CD8+ T and NK cells more than those from HSC-3 cells. However, this effect varied depending on the source and the immune and cancer cell subgroups. In zebrafish, the amount of IL-13 mRNA was decreased by SCC-25 EVs. This study describes promising in vitro and in vivo models to investigate interactions between immune cells, cancer cells, and EVs.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.