Time-course differential lncRNA and mRNA expressions in radioresistant hypopharyngeal cancer cells
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Jieyu Zhou1,2,*, Shengda Cao1,*, Wenming Li1, Dongmin Wei1, Zhentao Wang2, Guojun Li3,4, Xinliang Pan1 and Dapeng Lei1
1Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Key Laboratory of Otolaryngology, NHFPC - Shandong University, Jinan, Shandong, 250012, P.R. China
2Department of Otorhinolaryngology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, 200011, P.R. China
3Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA
4Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA
*These authors contributed equally to this work
Xinliang Pan, email: [email protected]
Dapeng Lei, email: [email protected]
Keywords: hypopharyngeal squamous cell carcinoma, radioresistance, lncRNA, mRNA, microarray
Received: December 05, 2016 Accepted: April 10, 2017 Published: April 21, 2017
Radioresistance remains a major problem in the treatment of patients with hypopharyngeal squamous cell carcinoma (HSCC). Long noncoding RNAs (lncRNAs) have important roles in the development, invasion, and metastasis of various tumors, including HSCC, but little is known about the role of lncRNAs in cancer radioresistance. The aim of this study was to identify radioresistance-related lncRNAs and mRNAs in radioresistant (RS) hypopharyngeal cancer subclone RS-FaDu cells. In this study, we performed microarray analysis to find the differences in time-course lncRNA and mRNA expression profiles between RS-FaDu and parent FaDu cells after 4 Gy radiation therapy, whose reliability was confirmed by validation experiment. Among these consistently dysregulated lncRNAs, we found that some lncRNAs (e.g., TCONS_00018436) might control resistance of HSCC cells to radiation. Furthermore, our bioinformatics analyses from mRNA/lncRNA microarray data showed that certain lncRNAs or mRNAs potentially are involved in radioresistance of HSCC. Our results from this study laid the foundation for further investigating the roles of these lncRNAs and mRNAs as promising candidates in the occurrence and development of HSCC radioresistance.
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