Oncotarget

Research Papers:

Xenogeneic human umbilical cord-derived mesenchymal stem cells reduce mortality in rats with acute respiratory distress syndrome complicated by sepsis

Fan-Yen Lee, Kuan-Hung Chen, Christopher Glenn Wallace, Pei-Hsun Sung, Jiunn-Jye Sheu, Sheng-Ying Chung, Yung-Lung Chen, Hung-I Lu, Sheung-Fat Ko, Cheuk-Kwan Sun, Hsin-Ju Chiang, Hsueh-Wen Chang, Mel S. Lee and Hon-Kan Yip _

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Oncotarget. 2017; 8:45626-45642. https://doi.org/10.18632/oncotarget.17320

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Abstract

Fan-Yen Lee1,*, Kuan-Hung Chen2,*, Christopher Glenn Wallace3, Pei-Hsun Sung4, Jiunn-Jye Sheu1, Sheng-Ying Chung4, Yung-Lung Chen4, Hung-I Lu1, Sheung-Fat Ko5, Cheuk-Kwan Sun6, Hsin-Ju Chiang7, Hsueh-Wen Chang8, Mel S. Lee9,** and Hon-Kan Yip4,10,11,12,13,**

1Division of Thoracic and Cardiovascular Surgery, Department of Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

2Department of Anesthesiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

3Department of Plastic Surgery, Royal Devon & Exeter Hospital, Exeter, United Kingdom

4Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

5Department of Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

6Department of Emergency Medicine, E-Da Hospital, I-Shou University School of Medicine for International Students, Kaohsiung, Taiwan

7Department of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

8Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan

9Department of Orthopedics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

10Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

11Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

12Department of Nursing, Asia University, Taichung, Taiwan

13Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan

*These authors have contributed equally to this work

**These authors have contributed equally to this work senior co-authors

Correspondence to:

Hon-Kan Yip, email: [email protected]

Keywords: acute respiratory distress syndrome, sepsis syndrome, inflammatory and immune reactions, xenogeneic mesenchymal stem cell, mortality

Received: January 10, 2017    Accepted: April 03, 2017    Published: April 21, 2017

ABSTRACT

This study tested the hypothesis that xenogeneic human umbilical cord-derived mesenchymal stem cell (HUCDMSC) therapy would improve survival rates in rats with acute respiratory distress-syndrome (ARDS, induction by 48 h inhalation of 100% oxygen) and sepsis-syndrome (SS, induction by cecal-ligation and puncture) (ARDS-SS). Adult-male Sprague-Dawley rats were categorized into group 1 (sham-controls), group 2 (ARDS-SS), group 3 [ARDS-SS+HUCDMSC (1.2 ×106 cells administered 1 h after SS-induction)], and group 4 [ARDS-SS+HUCDMSC (1.2 ×106 cells administered 24 h after SS-induction)]. The mortality rate was higher in groups 2 and 4 than in groups 1 and 3 (all p<0.0001). The blood pressure after 28 h was lower in groups 2, 3 and 4 (p<0.0001) than in group 1. Albumin levels and percentages of inflammatory cells in broncho-alveolar lavage fluid, and the percentages of inflammatory and immune cells in circulation, were lowest in group 1, highest in group 2, and higher in group 3 than group 4 (all p<0.0001). The percentages of inflammatory cells in ascites and kidney parenchyma showed identical patterns, as did kidney injury scores (all p<0.0001). EarlyHUCDMSC therapy reduced rodent mortality after induced ARDS-SS.


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