Oncotarget

Research Papers:

Analysis of STAT3 post-translational modifications (PTMs) in human prostate cancer with different Gleason Score

Rossana Cocchiola, Donatella Romaniello, Caterina Grillo, Fabio Altieri, Marcello Liberti, Fabio Massimo Magliocca, Silvia Chichiarelli, Ilaria Marrocco, Giuseppe Borgoni, Giacomo Perugia and Margherita Eufemi _

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Oncotarget. 2017; 8:42560-42570. https://doi.org/10.18632/oncotarget.17245

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Abstract

Rossana Cocchiola1,2,5,*, Donatella Romaniello1,2,*, Caterina Grillo1,2, Fabio Altieri1,2, Marcello Liberti3, Fabio Massimo Magliocca4, Silvia Chichiarelli1,2, Ilaria Marrocco1,2, Giuseppe Borgoni3, Giacomo Perugia3,* and Margherita Eufemi1,2,*

1Department of Biochemical Sciences, Sapienza University of Rome, Rome, Italy

2Istituto Pasteur, Fondazione Cenci Bolognetti, Piazzale Aldo Moro 5, Rome, Italy

3Department of Gynecological-Obstretic Science and Urologic Sciences, Sapienza University of Rome, Rome, Italy

4Department of Radiological, Oncological and Pathological Sciences, Sapienza University of Rome, Rome, Italy

5Fondazione Enrico ed Enrica Sovena, Rome, Italy

*These authors contributed equally to this work

Correspondence to:

Margherita Eufemi, email: margherita.eufemi@uniroma1.it

Keywords: STAT3, PTMs, signaling pathways, prostate cancer, biomarkers

Received: June 08, 2016     Accepted: April 05, 2017     Published: April 19, 2017

ABSTRACT

Prostate Cancer (PCa) is a complex and heterogeneous disease. The androgen receptor (AR) and the signal transducer and activator of transcription 3 (STAT3) could be effective targets for PCa therapy. STAT3, a cytoplasmatic latent transcription factor, is a hub protein for several oncogenic signalling pathways and up-regulates the expression of numerous genes involved in tumor cell proliferation, angiogenesis, metastasis and cell survival. STAT3 activity can be modulated by several Post-Translational Modifications (PTMs) which reflect particular cell conditions and may be implicated in PCa development and progression. The aim of this work was to analyze STAT3 PTMs at different tumor stages and their relationship with STAT3 cellular functions. For this purpose, sixty-five prostatectomy, Formalin-fixed paraffin-embedded (FFPE) specimens, classified with different Gleason Scores, were subjected to immunoblotting, immunofluorescence staining and RT-PCR analysis. All experiments were carried out in matched non-neoplastic and neoplastic tissues. Data obtained showed different STAT3 PTMs profiles among the analyzed tumor grades which correlate with differences in the amount and distribution of specific STAT3 interactors as well as the expression of STAT3 target genes. These results highlight the importance of PTMs as an additional biomarker for the exactly evaluation of the PCa stage and the optimal treatment of this disease.


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